摘要
本试验通过试验林自然发病调查、病根苗抗病性测定及ＤＡＰＩ染色等方法，对不同无性系及杂交组合的泡桐试材抗病性进行了评估。结果表明，５～６年生泡桐自然感染ＭＬＯ后的地面部分发病现象可归纳为四种类型：丛枝型（ＷＢ）、黄化型（Ｙ）、丛林黄化型（ＷＢ＋Ｙ）和丛枝花变叶型（ＷＢ＋Ｐ）。外部发病差异与其韧皮部筛管中ＭＬＯ的含量有直接联系。一般情况下，ＭＬＯ的浓度高低依次为花变叶部位、丛枝部位、黄化部位、无症或健康部位。在根部ＭＬＯ浓度低，且无性系间差异不显著。不同无性系间或杂交组合间抗病性存在明显差别。根据病状反应、病情指数和病组织ＭＬＯ浓度测定结果，可以区分为高抗、中抗和感病三种类型。初步筛选出一批抗病性强、生长性状好有可能被利用的试材，以及生长性状差但表现出较强抗丛枝病特性的无性系或杂交组合。
Resistance of the clones and hybrids in genus
Paulownia were evaltiated by field investigation and DAPI staining fluorescence microscopy for
MLO detection,Restilts showed that four types of symptoms were usually occurred in adult
diseased trees,i.e. I.witches' broom(WB),Ⅱ.yellowing(Y),Ⅲ.witches' broom &
yellowing(WB+Y),and Ⅳ.witches' broom &Phyllody,(WB+P).Symptom expression of diseased
trees are directlyrelevant to the MLO concentration within the sieve tubes of vascular tissues.In
general,theMLO concentration varies from high to low in order as
follows:phyllody,witches'broom,yellowing and healthy-like parts.However,MLO population in
the roots of all the cloneswere indistinguishable by means of DAPI staining fluorescence
microscopy. There wereapparent differences in the resistances of various clones and hybrids
to MLOs. According tothe types of symptoms,disease index and MLO detection results,the
testing material can bedivided into three categories:high-resistant,middle-resistant and
susceptible. Several goodclones and hybrids with high resistance and ideal growth character
were screened.
出处
《林业科学研究》
CSCD
北大核心
1994年第2期155-161,共7页
Forest Research
基金
"七五"国家攻关项目
关键词
泡桐无性系
类菌原体
抗病性
Paulownia clone,mycoplasma-like organism(MLO),resistance ).
