摘要
介绍放射性同位素标记核酸的方法，包括缺口平移法标记ＤＮＡ、引物延伸法标记ＤＮＡ、聚合酶法标记ＲＮＡ、Ｔ４多核苷酸激酶标记ＤＮＡ或ＲＮＡ的５′－末端、末端脱氧核苷酸基转移酶标记ＤＮＡ的３′－末端、ＤＮＡ聚合酶ⅠＫｌｅｎｏｗ片段标记ＤＮＡ的３′－末端、Ｔ４ＤＮＡ聚合酶标记ＤＮＡ的３′－末端、标记寡核苷酸。
Nucleic acid probe labelled with radioisotope is a useful tool in research of gene engineering and molecular biology. In this review, the methods for incorporating radioisotopes into nucleic acid were described. They include the nick-translation, primer extension, RNA polymerase-based methods, end-labelling ( 5′-end-labelling with T4 polynucleotide kinase, 3′-end-labelling with terminal deoxynucleotidyl transferase, end repair catalysed by Klenow polymerase, labelling with T4 DNA polymerase) , labelling of oligonucleotides.
出处
《核科学与工程》
CSCD
北大核心
1994年第3期280-283,共4页
Nuclear Science and Engineering
