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云南省鼠疫疫源地鼠疫菌质粒DNA种类及分子流行病学研究 被引量:38

Study on the Category of Plasmid DNA and Molecular Epidemiology of Yersinia pestis Isolated from Yunnan Natural Plague Foci
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摘要 用琼脂糖凝胶电泳技术,检查了分离自云南省的828株鼠疫菌质粒DNA特征。结果表明,云南鼠疫菌可观察到分子量为3.93、6.05、22.97、35.65、45.35、64.82、74.59、111.36和129.55Mdal九种质粒,按质粒组成可划分为Ⅰ~Ⅹ种质粒图谱。被检菌株中,99.15%具有6.05、45.35和64.82Mdal三种规范化质粒,还发现21.62%、4.59%、1.45%和3.5%的菌株分别尚存3.93、22.97、35.65和111.36Mdal质粒,这些质粒有特定的分布区域,具有重要的分子流行病学意义。根据质粒图谱,可将我省鼠疫现有疫区初步划分成滇西北山地、保山盆地、大盈江、陇川江、南定河、澜沧江下游和红河元江段流域七大相对独立的疫源区。本研究的结果结合既往流行病学资料,似可说明:①质粒组成可作为云南省鼠疫疫源地中疫源地划分的重要指标;②云南家、野鼠两型疫源地菌株具有共同的遗传变异性;③近几年之间鼠疫有复燃也存在异地传播;④云南西南部存在鼠疫疫源。 he plasmid DNA content of 828 Y. pestis strains isolated from Yunnan province were detected by agrosegel electrophoresis method. The result showed that these strains carried nine different kinds of plasmid withthe molecular weight about 3.93,6.05,22.97,35.65,45.35,64.82,74.59,111. 36 and 129. 55 Mdal,and ac-cording to the plasmid constituent of these strains,the plasmid profile can be divided into ten types. Amongthese strains, 99.15%strains have three normative plasmids with molecular weight about 6.05, 45.35,and64.82 Mdal,and 21.62%,4.59%,1.45%and 3.5%strains of the total existed 3.93, 22.97, 35.65 and111.36 Mdal plasmid respectively besides three normative plasmids, Those strains with non-normative plas-mids have a special region of distribution and it has an important moleculo─epidemiological significance.On thebasis of plasmid profile, our provincial pestilence district may be divided into seven relatively independentplague foci,namely,the northwest Yunnan mountain area,the Baoshan Basin,the Yingjiang river valley, theLongchuang river valley,the Nanding river valley,the Lancang river(Iower reaches) vaIley and the Yuanjiangsection of Honghe river.This study showed that plasmid content may be used as an important criterion in the division of middle plague foci in Yunnan plague foci. The Y.pestis strains isolated from domestic rat type and wild rodent type plague foci in Yunnan have a common character of heredity and mutation. In recent years,the epidemics may be due to resurgence but it may spread to other area also.There are natural plague foci existing in the sourth-weste Yunnan.
出处 《地方病通报》 1994年第4期58-63,共6页 Endemic Diseases Bulletin
基金 云南省青年科研基金
关键词 鼠疫菌 质粒图谱 流行病学 疫源地 Y. pestis Plasmid profile Molecular epidemiology Plague foci Yunnan province
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  • 1董兴齐,叶枫,彭何碧.鼠疫菌4Mdal质粒的发现及鉴定[J].中国地方病学杂志,1993,12(4):213-214. 被引量:10
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