摘要
本文对粗糙脉胞菌Neurosporacrassa1602摇瓶发酵产纤维素酶的条件进行了研究。结果表明:培养液初始pH5.5,培养温度28℃,摇瓶转速150r/min,培养96h,诱导产酶碳源为3%玉米芯粉+0.5%麸皮酸水解液(0.6mol/L),有机氮源为黄豆粉或无机氮源为NH4HSO4时为最佳产酶条件。其纤维素酶活力CMCase为10.34IU/ml,FPase为0.71IU/ml。
In this paper,fermentation conditions of cellulase produced by Neurospora crassa in the shaking bottle were studied. The results showed that the optimum conditions were as follows: cultivating solution initial pH was 5.5, cultivating temperature 28℃, rotation speed 150r/min and cultivating time 96 h. The most suitable organic nitrogen source was soybean powder and the most suitable inorganic nitrogen source was NH4HSO4. It helped produc cellulase under the conditions of 3% corncob powder and 0.5% bran acid hydrolysis solution(0.6 mol/L).In the optimum conditions, CMCase activity was 10.34IU/ml and FPase was 0.71IU/ml.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第1期67-70,共4页
Food Science
基金
天津市科委资助项目(043611111)
关键词
粗糙脉胞菌
纤维素酶
发酵
条件
Neurospora crassa
cellulase
fermentation
condition
