摘要
目的 :观察慢性复合应激对大鼠学习与记忆的影响和海马内NMDA受体亚基NR1表达的变化。方法 :成年雄性Wistar大鼠实验组每天交替暴露于复合应激原环境中达 6w ,然后作Morris水迷宫和Y迷宫作业测试 ,再采用免疫组织化学和图像处理方法分析海马CA1、CA3和齿状回区内NR1的表达变化。结果 :慢性复合应激组大鼠寻找平台的潜伏期较对照组明显缩短 ,学会躲避电击的正确次数较对照组明显增多 ;海马内NMDA受体亚基NR1的表达水平较对照组明显上调。结论 :慢性复合应激可增强学习与记忆能力 。
Objective: To study the effects of chronic complex stress on learning and memory and NMDA receptor subunits NR1 expression in hippocampus of rats. Methods: Wistar male rats were randomly divided into control group and experimental group. The rats in experimental group were alternatively exposed to chronic complex stress for 6 weeks. After completion of the stress for experimental rats, both groups were tested with Morris water maze and followed with Y-maze. Afterwards, the brain tissue section were stained immunohistochemically to detect the expression changes of NR1 and the immunoreactivity within the CA1, CA3 and dentate gyrus of the hippocampus and was analyzed by computer-assisted image analysis system. Results: Compared with control group, the latent periods to search platform was significantly shortened in experimental rats and the times to correctly avoid the electric stroke was significantly increased in the experimental rats; the expression of NR1 was significantly up-regulated. Conclusion: Chronic complex stress might increase the ability of learning and memory. One of these mechanisms might be related with the expression changes of NMDA receptors.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2004年第6期616-619,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金 (30 2 70 4 4 6 )
