摘要
目的:探讨氯沙坦对高糖培养的系膜细胞产生活性氧和血管内皮细胞生长因子(VEGF)mRNA表达的影响。方法:体外培养人系膜细胞,用高糖、氯沙坦干预不同时间后,应用比色法检测培养细胞上清液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活力,丙二醛(MDA)的水平。用RT鄄PCR法测定血管内皮细胞生长因子(VEGF)mRNA表达的影响。结果:与正常对照组相比,高糖组SOD、CAT的活力明显降低,而MDA含量明显升高,VEGFmRNA表达升高。与高糖组相比,高糖加氯沙坦组的SOD、CAT的活力升高,MDA水平下降,VEGFmRNA表达上调。结论:氯沙坦能抑制高糖所致细胞活性氧的产生和VEGF的表达,从而抑制细胞通透性升高,降低蛋白尿,发挥其肾保护作用。
Objective: To investigate the effect of Losartan on the production of reactive oxygen species(ROS) and the expression of endothelial vascular growth factor(VEGF) in human mesangial cells cultured in high glucose. Methods:Human mesangial cells were cultured in high glucose with or without Losartan, the activities of SOD and CAT, the level of MDA in the supernatant were measured by spectrophotometry and the expression of VEGF mRNA in the cells were measured by RT-PCR. Results:Compared with the control group, the activities of SOD and CAT were markedly lower, while the level of MDA was greatly higher in the high glucose group. High glucose stimulated the expression of VEGF mRNA. Compared with the high glucose group, high glucose with Losartan heightened the activities of SOD and CAT, declined the level of MDA, and down-regulated the expression of VEGF mRNA. Conclusion:Losartan might inhibit the production of ROS and down-regulate the expression of VEGF mRNA induced by high glucose. Thus the celluar permeability was suppressed, and the proteinuria was reduced. This maybe another mechanism which protects renal function from diabetic nephropathy .
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期150-153,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金资助项目(30370658)
关键词
氯沙坦
系膜细胞
活性氧
血管内皮细胞生长因子
Losartan
mesangial cell
reactive oxygen species
vascular endothelial growth factor
