摘要
目的 :观察川芎嗪联合氨基胍对糖尿病大鼠肾脏血管内皮细胞生长因子 (vascularendothelialgrowthfactor,VEGF)表达的影响 ,探讨川芎嗪联合氨基胍治疗对糖尿病肾脏病变的保护作用。方法 :用链脲佐菌素制作糖尿病大鼠模型 ,分为正常对照组、糖尿病模型组、川芎嗪治疗组、氨基胍治疗组和川芎嗪联合氨基胍治疗组 ,分别于第 12周用免疫组化法观察各组大鼠肾皮质VEGF表达的变化。结果 :糖尿病大鼠较正常大鼠肾皮质VEGF的表达明显增强 ,在肾小球上皮细胞尤为明显 ;川芎嗪治疗组和氨基胍治疗组大鼠肾皮质VEGF表达比未治疗组明显减弱 ,但仍高于正常对照组 ;川芎嗪联合氨基胍治疗组肾皮质VEGF的表达基本正常。结论 :川芎嗪联合氨基胍治疗可抑制糖尿病大鼠肾脏VEGF的过度表达 。
Objective:To investigate the therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy. Methods:Diabetic rats were induced by streptozotocin. They were divided into 5 groups: normal control group (group C), untreated diabetic group (group DM), tetromethylpyrazine treated group (group TMP), aminoguanidine treated group (group AG) and tetromethylpyrazine and aminoguanidine treated group (group TMP+AG). The expression of vascular endothelial growth factor (VEGF) in renal cortex of the rats in each group was observed by immunohistochemical staining after 12 weeks. Results:The expression of VEGF in renal cortex of the rats in group TMP+AG and group C was alike. The expression of VEGF in renal cortex of group TMP and group AG decreased significantly as compared with that of group C, but was still above normal level. Conclusion:The therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy may be inhibiting the over-expression of VEGF in kidney of diabetic rats.
出处
《中西医结合学报》
CAS
2004年第1期39-41,共3页
Journal of Chinese Integrative Medicine
关键词
川芎嗪
氨基胍
糖尿病
大鼠
肾脏
血管内皮细胞生长因子
治疗
tetromethylpyrazine
aminoguanidine
diabetic nephropathy
vascular endothelial growth factor
