摘要
目的:研究糖尿病大鼠肾脏组织中细胞外基质金属蛋白酶诱导因子(EMMPR IN)和基质金属蛋白酶2(MMP-2)的表达,探讨糖尿病肾病的发病机制。方法:将SD大鼠随机分成正常对照组(NC组)和糖尿病模型组(DM组),DM组腹腔注射链脲佐菌素(55 mg.kg-1)诱导糖尿病大鼠模型,于第4、第8周分批处死大鼠,用免疫组化检测EMMPR IN、MMP-2表达,图像分析仪分析。结果:正常大鼠EMMPR IN、MMP-2主要表达在肾小球系膜细胞、内皮细胞、足细胞、球囊壁层上皮细胞、肾小管上皮细胞,造模后4、8周时DM组大鼠EMMPR IN、MMP-2表达较正常对照组明显下降。结论:糖尿病时肾小球EMMPR IN和MMP-2表达水平的降低,可能是引起细胞外基质积聚的原因之一。
Objective To investigate the expression of extracellular matrix metalloproteinase inducer （EMMPRIN） and matrix metalloproteinase-2 （MMP-2） in diabetic rat＇ s kidneys. Methods Rat diabetic model was induced by streptozotocin （55 mg · kg ^-1 ） , and kidneys were examined pathologically and the expression of EMMPRIN and MMP-2 was studied by immunohistochemistry. The intensity of EMMPRIN and MMP-2 was analyzed by imaging quantitative a- nalysis technique. Results EMMPRIN and MMP-2 were mainly expressed in the mesangial cells, endothelial cells, podocytes, parietal epithelial cells of Bowman＇ s capsule, and tubular cells. The expression of EMMPRIN and MMP-2 was significantly weakened in the glomeruli of diabetic rats than that of the control animals （ P 〈 0.05 ）. Conclusion Expression of EMMPRIN and MMP-2 in glomeruli is markedly decreased in diabetic rats, which is involved in the development of diabetic nephropathy .
出处
《东南大学学报（医学版）》
CAS
2006年第3期159-163,共5页
Journal of Southeast University(Medical Science Edition)
基金
国家自然科学基金资助项目(30370658)
关键词
糖尿病
细胞外基质金属蛋白酶诱导因子
基质金属蛋白酶2
表达
大鼠
diabetic mellitus
extracellular matrix metalloproteinase inducer
matrix metalloproteinase- 2
expression
rats
