摘要
目的 探讨散发性Burkitt淋巴瘤 (BL)的临床病理和免疫表型特征。方法 对 6例散发性BL病例进行了光镜、免疫组化及原位分子杂交检测 ,再结合临床特征进行临床病理分析。结果 6例散发性BL中 ,男性 5例 ,女性 1例 ;其中青少年占 4例。 4例表现为颌下包块 ,2例表现为颈部包块。光镜下BL瘤细胞呈弥漫一致的中等大细胞 ,常伴有“星空现象”和多量的核碎片 ,易见核分裂象。免疫表型 :瘤细胞示CD2 0 +和 (或 )CD79α +,Ki 6 7 ,bcl 2、TdT和MPO均 - ;原位杂交显示E BER +(1/3例 )。结论 散发性BL属高度恶性淋巴瘤 ,镜下瘤细胞表现为弥漫一致的中等大细胞 ,染色质粗 ,可见少量胞质 ,核分裂及核碎片多见 ;常伴“星空现象” ;免疫组化示B细胞性 ,Ki 6 7接近 10 0 %。BL主要应与淋巴母细胞性淋巴瘤。
Purpose To investigate the clinical and pathologic features of the sporadic Burkitt's lymphoma (BL), as well as its immunohistochemical characteristics. Methods Six cases of sporadic BL were studied by light microscopy, immunohistochemistry and in stiu hybridization, and analyzed with clinical features. Results Sporadic BL occurred in 4/6 cases of children and young adults, 5 cases in male, with submandicular mass in 4 cases and cervical mass in 2 cases. Morphologically, the tumor was characterized by a monotonous infiltration of medium-sized cells, usually with a “starry-sky” pattern and numerous karyorrhectic debris. Mitotic figures were frequent. Immunohistochemically, the tumor cells were positive for CD20 and/or CD79α, and negative for bcl-2, TdT and MPO. The index of Ki-67 was nearly 100%. EBER was positive in 1/3 case by in situ hybridization. Conclusions Sporadic BL is a high-grade malignant B cell lymphoma, which presents following features: diffuse monotonous infiltration of medium-sized cells with coarse chromatin and an appreciable rim of basophilic cytoplasm. The mitotic figures and karyorrhectic debris are frequent; usually with a “starry-sky” pattern; the tumor cells are positive for B cell markers, and Ki-67 index is very high (nearly 100%) by immunohistochemistry. The main differential diagnoses include lymphoblastic lymphoma, myeloid leukemia, and nonhematopoietic small cell neoplasms.
出处
《临床与实验病理学杂志》
CAS
CSCD
2004年第6期666-669,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
淋巴瘤
临床病理
免疫组织化学
诊断
鉴别诊断
lymphoma
clinicopathologic features
immunohistochemistry
diagnosis
differential diagnosis
