摘要
目的:对大鼠C6胶质瘤细胞应用实时荧光定量PCR(real-time quantitative PCR,q PCR)法观察白细胞介素-12(Interleukin-12,IL-12)家族亚基基因的表达,为今后IL-12家族在脑胶质瘤方面研究奠定基础。方法:提取大鼠C6胶质瘤细胞RNA,反转录成c DNA,应用q PCR法观察IL-12家族亚基基因在大鼠C6胶质瘤细胞mRNA表达影响并进行比较分析。结果:在大鼠C6胶质瘤细胞中,IL-23a,IL-12a的表达丰度为高,EBI3,IL-27的表达丰度为中,IL-12b的表达丰度为低。结论:IL-12家族与胶质瘤发生、发展密切相关,其中IL-12、IL-23是最有潜力治疗脑胶质瘤的细胞因子,其研究进展将为脑胶质瘤的免疫治疗带来新思路。
Objective：To observe the expression of IL-12 family subunit genes by real-time quantitative PCR in mice C6 glioma cells, construct the basis of the brain glioma research on IL-12 family in the future. Methods： Mice C6 glioma RNA was abstracted and reversed transcription cDNA. The mice C6 glioma ceils mRNA expression influence of IL-12 family subunit genes was compared and analyzed by real-time quantitative PCR. Results： In mice C6 glioma cells, high expression abundances in IL-23a, IL-12a, midlde expression abundances in EBI3, IL-27, low expression abundance in IL-12b. Conclusion： IL-12 families are closely related to the occurrence and development of glioma, IL-12,IL-23 are regarded as the most potential anti-glioma eytokines among them, research de- velopments will bring a new way of brain glioma immune therapy.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第5期600-602,606,共4页
Chinese Journal of Immunology
基金
2011年国家自然科学基金项目(81172394)
2014年厦门市科技局基金项目(3502Z20144016)
