摘要
目的 比较两种方法制备的猪脱细胞真皮基质分别与自体刃厚皮复合移植修复大鼠全层皮肤缺损的效果。方法 使用 Dispase /Triton X 10 0 (中性蛋白酶 /曲拉通 )和高渗盐水 /十二烷基硫酸钠 (SDS)两种方法去除猪表皮及真皮中的细胞成分 ,分别得到猪脱细胞真皮基质 和 。 6 3只 SD大鼠背部全层皮肤缺损分别使用猪脱细胞真皮基质 +自体刃厚皮及猪脱细胞真皮基质 +自体刃厚皮覆盖 ,术后观察移植物成活率和植皮区收缩率 ,同时取移植物进行组织学观察 ,并与单纯自体刃厚皮移植相比较。结果 两种方法制备的猪脱细胞真皮基质分别与自体刃厚皮复合移植的移植物成活率和植皮区收缩率差异无显著性 ,组织学观察显示复合皮上皮化良好 ,胶原纤维排列有序 ,基底膜结构完整。两复合皮组术后第 6周移植物成活率与自体刃厚皮组比较差异均无显著性 ,术后第 4周开始两复合皮组移植物收缩率明显降低。结论 两种方法制备的异种脱细胞真皮基质与自体皮复合移植都能很好地修复全层皮肤缺损 ,改善创面愈合质量。
Objective To observed the effect of healing quality of composite skin grafting consisting of acellular porcine dermal matrix combined with autologous splitthickness skin graft. Methods Porcine skin was treated with dispaseⅡ/triton X100 or hyperosmotic saline/sodiumdodecylsulfate(SDS) respectively , and acellular porcine dermal matrixⅠ(APDMⅠ) and APDMⅡ were obtained. Sixtythree SpragueDawley rats with fullthickness skin defects on the back were separately covered with APDMⅠ+splitthickness autologous skin, or APDMⅡ+splitthickness autologous skin. The quality of wound healing was observed, the rates of survival and contraction of the grafts were calculated, the tissue samples were harvested for histological examination, and compared with that of autologous splitthickness skin graft. Results The wound healing quality of composite skinⅠ, and Ⅱwas good. There was no significant difference in the rate of survival and contraction of the grafts between the two composite skin grafting groups. It was indicated by histological examination intact basal membrane. There was no significant difference in the survival rate between composite skin grafting groups and autologous splitthickness skin at the 6 th week after operation, but the contraction rates of the grafts in the composite skin groups were lower . Conclusion Fullthickness skin defect can be healed by covering with acellular porcine dermal matrix produced by two methods combined with splitthickness autolograft, and it can help improve the quality of wound healing.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2005年第2期92-94,F007,共4页
Chinese Critical Care Medicine
基金
首都医学发展科研基金重点项目 (2 0 0 2 2 0 3 6)
全军临床高新技术重大项目 (2 0 0 2 3 5 )
关键词
猪脱细胞
真皮基质
创面移植
复合移植
皮肤移植
acellular porcine dermal matrix
autologous split-thickness skin
skin transplantation
composite skin transplantation
