摘要
针对脱细胞真皮基质作为复合移植用真皮支架所存在的渗透性较差、降解速率慢、生物活性低等缺点,采用新工艺技术制备了天然三维网络结构的新型胶原支架材料,探讨交联、复合及其顺序和打孔对其理化性能的影响,并通过成纤维细胞培养试验研究其生物相容性。理化性能检测结果显示:复合提高交联试样的孔隙率和延长降解时间;交联明显提高试样的孔隙率和拉伸强度并延长其降解时间;先复合后交联可以提高孔隙率和降解时间;打孔可以明显提高材料的透水汽性和孔隙率。成纤维细胞培养试验表明:成纤维细胞能在改性支架材料上黏附、增殖。新型胶原支架经复合、交联、打孔改性后,其透水汽性明显提高,达到(3372±83)g/(m2·d);孔隙率显著提高,达(94.20±2.5)%;拉伸强度较好,为(10.65±0.32)MPa;体外降解时间为(38.3±1.0)h,通过适当改变交联剂的用量在比较宽的范围,可以满足不同支架材料降解速率的要求;细胞相容性良好。新型胶原支架经复合、交联、打孔改性后有望作为复合移植用的真皮支架。
In order to overcome the shortcomings of acellular dermal matrix which was used for composite grafting, such as bad permeability, low biological activity and so on, a dimensional meshwork collagen dermal matrix was prepared by a firenew technology. The modifcation methodls of cross-linkage, complex, perforating and fibroblast cultivation were mainly discussed in this paper. The results showed that complex improved the porosity of cross-linkage samples and prolong its degradation time. Cross-linkage improved the porosity, tensile-strength and prolong its degradation time. The porosity and degradation time were improved when the processing sequence was complex before cross-linkage. Perforating improved the permeability and porosity obviously. The fibroblast cultivation revealed that the cells could well adhere, grow and multiplicate on the surface of modified materials. By using of modifications of complex, cross-linkage and perforating, the performance of new collagen scaffold was improved obviously. The permeability was （ 3372 ± 83 ） g/（ m^2 · d ）. The degradation time was （ 38.3 ± 1.0 ） h and could be controlled by the dosage of cross-linkage, in orden to satisfy the requests of different scaffolds. The porosity was increased evidently, to （94. 20 ± 2. 5 ）%. The tensile-strength was at （10. 65 ± 0. 32）MPa. The biocompatibility was improved as well. The results above indicated that this new collagen dermal scaffold would be suitable for composite graft in future.
出处
《北京生物医学工程》
2008年第6期617-620,636,共5页
Beijing Biomedical Engineering
基金
中国博士后科学基金(20070421074)
山东省自然科学基金重点项目(Z2003C01)
山东省科技发展计划项目(2008GG1002031)资助
关键词
胶原
支架材料
改性
生物改性
制备技术
collagen
scaffold materials
modification biological activity
preparation technology
