摘要
培养基组成成分能影响人参培养细胞单细胞克隆的植板率。ＭＳ培养基中２，４－Ｄ和ＫＴ需要一个适合的浓度比才能有效地促进细胞克隆的形成，其最佳浓度组合是２，４－Ｄ１．５ｍｇ／Ｌ和ＫＴ ０．５ｍｇ／Ｌ。适合人参细胞克隆形成的ＮＨ４ＮＯ３浓度是４０ｍｇ／Ｌ，ＣａＣｌ２·２Ｈ２Ｏ浓度是７５０ｍｇ／Ｌ。向培养基中补加适量的琥珀酸、精氨酸和维生素等都能明显提高植板率。通过优化培养基的组成成分。
The plating efficiency of single cell clone from culture cells of Panax ginseng could be influenced by the nutritional compositions of plating medium. In order to promote the growth of single cells, it was essential to establish a appropriate ratio between 2,4-D concentration and KT concentration. The best combination of these two hormones was 1.5mg/L and 0.5mg/L, respectively. Considering the formation of cell clones, the suitable NH_4NO_3 concentration and CaCl_2·2H_2O concentration should be 400mg/L and 750mg/L, respectively. The plating efficiency could also be effectively increased when the plating medium was suitably supplemented with succinate acid, arginine, inositol and so on. Optimization of the nutritional compositions of medium resulted in plating efficiency close to 2.34 times against controlled. The cells suspensed for 12 days was the most favourable for the formation of cell clones. Different plating efficiency was obtained with different cell plating density. There was a low plating efficiency when the cells plating density was no more than 4×10~3cells/ml.
出处
《生物工程学报》
CAS
CSCD
北大核心
1993年第4期337-341,共5页
Chinese Journal of Biotechnology
基金
国家自然科学资金
关键词
人参
单细胞克隆
平板培养
细胞
Panax ginseng
single cell clone
plating culture
plating efficiency(PE)
