摘要
高产花色苷玫瑰茄细胞系的筛选杜金华郭勇（华南理工大学生物工程研究所广州５１０６４１）花色苷在植物中呈现粉红、红、紫红、紫等颜色，可以用作食品、药品及化妆品的着色剂，亦有药用价值。作为食品添加剂，颜色较合成色素自然，且安全无毒性。早在１９８７年，Ｍｉｚ...
High production anthocyaninsroselle ( Hibiscus sabdariffa L.) cell lines were selected by both single cell clone plate culture and small cell aggregate culture. The cell suspension cultures for 8 days were most favourable for the formation of cell clones. When pH value was 5.84 as well as CaCl 2·2H 2O concentration was 750mg/L of B 5 medium, the plating efficiency (PE) could be intensively increased. There was a low PE when the cell plating density was no more than 6×10 3/ml. Homogenous and dark color samll cell aggregates were selected in high conten pigment areas of roselle callus and cultivated in B 5 medium (pH5.84; CaCl 2·2H 2O:750mg/L). Both red cell lines and cell aggregates selected from cultures were subcuhured succesfully for 10 generations. The anthocyanin content in high production cell line was 2.33% (g/g dw.), as high as 14.5 times of the control.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第4期437-439,共3页
Chinese Journal of Biotechnology
关键词
玫瑰茄
锦葵科
细胞系
花色苷
Roselle ( Hibiscus sabdariffa L.)
cultured cell
anthocyanin
selection
