摘要
利用东北红豆杉叶片愈伤组织细胞为材料，进行了细胞克隆技术的研究。发现悬浮培养细胞第３代的植板率最高，ｐｈｙｔａｇｅｌ与低熔点胶的植板率相当，固体－固体双层培养可适当提高细胞的植板率。通过东北红豆杉细胞克隆的平板培养，获得了一批速生、稳定的细胞系，生长速率最高可达０．４ｇＦＷ／ｇＦＷｄ，完全克服了红豆杉细胞培养过程中的褐化现象。
The cell clone technique was studied for Taxus cuspidata with needlederived calli cells.The plating efficiency(PE)of the third generation of suspension culture cells was found to be the highest one and the PE of the medium solidified with phytagel was found to be almost the same as that of the medium solidified with low melting agarose gel.Solidsolid double layer culture was found to promote the PE of Taxus cuspidata cells moderately.A large number of stable cell lines with high growth rate were obained and the browning problem of cells was completely solved via plate culture of the cell clones of T.cuspidata.The growth rate of the best cell line reached 0.4g.FW/g.FW.d.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1998年第5期265-267,共3页
China Journal of Chinese Materia Medica
关键词
东北红豆杉
细胞克隆
平板培养
植板率
Taxus cuspidata
cell clone
plate culture
plating effeciency
