摘要
溶菌酶(MW～14KD)是一种水解细菌细胞壁多糖的水解酶.危害严重的职业病矽肺患者血清溶菌酶显著升高.本文报道,采用高效液相色谱技术,包括凝胶过滤柱层析和反相C4高效液相色谱柱层析,从实验性矽肺大鼠的肺泡灌洗液(BALF),肺泡巨噬细胞裂解液(AME)及其条件培养液(AMCM)中,分离纯化了一种可诱导的巨噬细胞来源的细胞因子(iSPMF＿P15),其分子量约为15 4KD,它具有较明显的刺激成纤维细胞增殖的作用.因此,矽肺肺泡巨噬细胞来源的溶菌酶可能是矽肺纤维化病变中的一种致纤维化的细胞因子.
Lysozyme is a hydrolase, which is able to hydrolyze the wall polysaccharides of bacteria. It is known that silicosis patients display a high level of serum lysozyme. The present work reports that an inducible silicotic (rat)pulmonary macrophage-derived factor (iSPMF-P^(15)) was isolated from the broncho-alveolar lavage, alveolar macrophages and its culture medium of silicotic rats. The final purified iSPMF-P^(15) had a molecular weight of 15400 and is identified as lysozyme. The silicotic macrophage-derived lysozyme was capable of stimulating the fibroblast proliferation.
出处
《中央民族大学学报（自然科学版）》
2004年第4期357-359,379,共4页
Journal of Minzu University of China(Natural Sciences Edition)
