摘要
目的分离、鉴定与矽肺发生、发展过程相关的几种蛋白质，并测定其促成纤维细胞增殖的活性。方法采用高压液相色谱技术包括凝胶过滤柱层析、离子交换柱层析、反相Ｃ４高压液相色谱柱层析，分别对矽肺大鼠肺泡灌洗液（ＢＡＬＦ）、肺泡巨噬细胞冻融物（ＡＭＥ）及其条件培养液（ＡＭＣＭ）进行分离纯化，并通过Ｎ末端氨基酸序列的测定来鉴定这些蛋白质。结果（１）染尘２１天后，矽肺大鼠ＢＡＬＦ中白蛋白含量明显升高；（２）从ＢＡＬＦ、ＡＭＥ和ＡＭＣＭ中除纯化到相对分子质量为１５４００的溶菌酶外（其刺激成纤维细胞增殖的活性为１４０％），还纯化到一种新的具有明显促成纤维细胞增殖的细胞因子（Ｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｐｒｏｍｏｔｉｎｇｆａｃｔｏｒ，ＦＧＰＦ），其最适作用浓度为２．０～６．０μｇ／ｍｌ。结论巨噬细胞分泌的ＦＧＰＦ和溶菌酶在矽肺纤维化过程中可能有重要作用。
Objective To find out some of the fibrogenic
factors occurred in bronchoalveolar lavage fluid(BALF) of rats,which are related to the formation
of silicosis. Methods A series of high performance liquid chromatograph(HPLC)
techniques,including gel permeation,ion exchange and reversephase HPLC were carried out.
Results Several proteins were purified from BALF,freeze thawed alveolar macrophage
extract(AME) and alveolar macrophage cultured medium(AMCM) of the silicosis rats.There were
significant increase of albumin and lysozyme.The latter showed 15 400 of molecular weight
with 140% of fibroblast growth stimulating activity.In addition,a novel protein (58 000) with low
homogeneity in terminal amino acid sequences and potent fibrogenic activity was discovered in
this study. ConclusionBZThe factor named fibroblast growth promoting factor(FGPF) and
lysozyme may play important role in fibroblast proliferation during the process of silica induced
fibrosis.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1999年第3期151-154,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
