摘要
低成本纤维素酶的生产是酶法转化纤维质生物量为酒精的关键。尽管已有很多有关纤维素酶的研究报告,但底物的预处理对固态发酵过程中pH值和产酶的影响很少见诸于报道。作者研究了在固态发酵过程中,里氏木霉在未处理和经挤压处理的麦糟培养基上pH值的变化。经单螺杆和双螺杆挤压的麦糟,其纤维的结晶度变化较小。单螺杆挤压撕开了纤维结构,而双螺杆破坏并摧毁了纤维细胞的细胞壁,同时麦糟的颗粒也变得很小。两种经挤压处理的麦糟均有利于菌体的生长,并提高了产酶。以单螺杆挤压处理的麦糟为培养基时,最高FPA酶活力为182.8IU/g纤维素。
The production of low-cost cellulase enzyme is a key step in the development of an enzymatic-based process for conversion of lignocellulosic biomass to ethanol.Although abundant informations are available on cellulase production,little work has been examined on the effects of substrate pretreatment on pH value and cellulase formation during solid-substrate fermentation.This paper investigated pH value changes of substrate during fermentation of Trichoderma reesei on native and extruded substrates.After single-screw and twin-screw extrusion,cellulose crystallinity of spent grain was reduced slightly.Single extrusion fragmented and opened cellulose structure,while twin extrusion destroyed and disrupted the cell wall structure of spent grain as well as reduced particle size.Both extrusions favor micro-(organism) growth and enhance the production of cellulase.Maximal FPA activity of 182.8 IU/g cellulose was observed when spent grain by single extrusion was used as substrate.
出处
《林产化学与工业》
EI
CAS
CSCD
2004年第4期81-86,共6页
Chemistry and Industry of Forest Products
关键词
固态发酵
麦糟
纤维素酶
里氏木霉
solid-substrate fermentation
spent grain
cellulase
Trichoderma reesei
