摘要
根据大肠杆菌O15 7:H7特异性基因fliC ,rfbE ,SLTI与SLTII设计四对引物 ,建立了复合PCR方法 ,扩增产物长度分别为为 5 6 0bp ,6 78bp ,2 10bp与 4 84bp。该方法可以一步检测出O15 7与H7特异性抗原基因 ,并可同时检测该菌产生SLT毒素的类型 ,与 71株试验菌株的基因型完全配合 ,是一种特异、高效的检测方法。该方法适用于牛奶样品中大肠杆菌O15 7:H7的检测 ,经过增菌步骤检测限可达 0 1cfu
A multiplex PCR assay for detection of Escherichia coli O157:H7 was developed with 4 pairs primers which focus on its specific fliC,rfbE,SLT I and SLT II genes.The target genes fragments of the PCR assay were 560bp,678bp,210bp and 484bp.The multiplex PCR had very high specificity to match all the 71 tested strains including 58 E.coli strains and other 18 strains.The strain of E.coli O157:H7 was identified in one step,and the type of SLT was also identified as well.With the enrichment procedure,the strain of E.coli O157:H7 were recovered at the detection limit of 0.1 cfu/ml from artifical inoculated pasteurized milk.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第6期716-719,共4页
Journal of Hygiene Research
基金
国家科技部十五攻关项目 (No.2 0 0 1BA80 4A0 3)
