摘要
观察IL - 1对髁状突软骨 (Mandibularcondylarcartilage ,MCC)细胞增殖及凋亡的影响。 方法下颌骨髁状突软骨取自 6只新生的日本大耳白兔 ,经机械分离及胰蛋白酶、胶原酶联合消化获得MCC细胞。取第2代软骨细胞 ,培养过程中培养液中加入不同浓度 (0 .1μg/L、10 0 μg/L)的IL - 1,采用相差显微镜、流式细胞术及透射电镜对髁状突软骨细胞生物学行为变化进行研究。结果 IL - 1在 1μg/L～ 10 0 μg/L范围内 ,呈剂量效应关系抑制髁状突软骨细胞增殖 ,流式细胞术结果表明MCC细胞多数阻断于G0 /G1期 ,S期细胞比例显著低于对照组(P <0 .0 5 )。同时 10 μg/L及 10 0 μg/LIL - 1可显著诱导细胞凋亡 (凋亡率分别为5 9.47%及 6 3 .73 % ) ,细胞收缩 ,形态变圆。电镜下观 ,胞浆浓缩 ,染色质边集 ,粗面内质网扩张。结论 IL - 1抑制MCC的细胞增殖 ,诱导细胞凋亡的发生 ,可能是其在骨关节病发病中重要作用途径之一。
Objective To investigate the influence of IL-1 on proliferation and apoptosis of rabbit mandibular condylar cartilage (MCC) cells.Methods MCC cells of white rabbits were harvested enzymatically and cultured in DMEM supplemented with 0.4% newborn calf serum.In the experiment,varying concentrations of IL-1 was added to the medium.The effects of IL-1 were assessed by means of MTT colorimetric method,cell cycle analysis with flow cytometry assay,and morphological observation with the phase contrast microscope observation and transmission electronic microscope.Results IL-1 can significantly inhibit MCC cells proliferation in a dose-dependent manner.In addition,IL-1 (10μg/L～100μg/L) promote the apoptosis of MCC cells.Cells shrank to rounded shape from polygonal.Ultrastructural examination revealed that condensation of cell plasma and many vacuoles presented in the apopotatic cells.Conclusion Being a key mediator of osteoarthritic cartilage destruction,IL-1 can inhibit the chondrocytes proliferation and induce apoptosis.This may be one of its pathogenic effects implicated in osteoarthrosis(OA).
出处
《现代口腔医学杂志》
CAS
CSCD
2000年第3期145-147,共3页
Journal of Modern Stomatology
基金
国家自然科学基金资助课题! (编号 :39770 80 0 )
