摘要
目的:研究体外培养的正常人胚髁状突软骨细胞的生物学特性.方法:体外培养人胚髁状突软骨细胞,观察其从原代至第6代的形态变化;测定细胞数量的变化及其生长曲线;测定冷冻复苏后的细胞存活;观察细胞的超微结构.结果:从原代至第3代,髁状突软骨细胞为圆形、多角形,从第4代开始逐渐转变为梭形的成纤维细胞.体外培养至第6天髁状突软骨细胞的数量是接种时的3倍.冷冻复苏后经苔盼蓝拒染试验检查,发现细胞存活率为92%.体外培养髁状突软骨细胞的超微结构正常.结论:单层培养髁状突软骨细胞的表型至少能够保持3代稳定,且形态结构正常,具有正常的生长增殖能力,并可经深低温冷冻长期保存.
Objective- The purpose was to study the biological characteristics of the condylar chondrocytes. Methods; The condylar chondrocytes of the normal fetal human were cultured in vitro. The changes of cellular morphology from primary to sixth subculture and the ultrastructure of the condylar chondrocytes were investigated. The growth curve and the survive rate of the condylar chondrocytes after being frozen (196℃)were studied. Results; The morphology of the condylar chondrocytes were maintained the sphercal and polygonal shape from the first to the third generation. but transformed into the shape of the fibrobiast cell after the fourth generation. The number of the condylar chondrocytes after being cultured in vitro for six days was approximately three fold of that of the plating. The survive rate of the condylar chondrocytes suscitating from being frozen(196℃) was 92%. The ultrastructure of condylar chondrocytes cultured in vitro was normal. Conclusion: In the monolayer culture, condylar chondrocytes maintainde the cartilage specific phenotype, the normal structure and the ability of reproduction in the first three generations. The condylar articular chondrocytes could survive the deep-frozen.
出处
《现代口腔医学杂志》
CAS
CSCD
1997年第4期241-244,共4页
Journal of Modern Stomatology
