摘要
目的 研究腹腔镜子宫切除术 (LH)和开腹子宫切除术 (AH)对免疫功能的影响。方法 2 0 0 1年 7月～ 2 0 0 3年 7月在因子宫肌瘤行LH患者 78例 ,对照组开腹手术的患者 4 2例。术前 2 4h、术后 2 4h及72h分别抽取肘静脉血 ,采用ELISA双抗体夹心法测定血清IL - 6、TNF -α ,用散射比浊法测定血清CRP水平 ;用流式细胞仪测定CD3+(T细胞总数 )、CD4 +(T辅助 /诱导细胞 )、CD8+(T抑制 /杀伤细胞 )、CD16 +(NK细胞 )数量。结果 手术前两组患者血清IL - 6、TNF -α和CRP的水平差异无显著性 (P >0 0 5 )。术后 2 4h、 72h两组患者的血清IL - 6、TNF -α和CRP水平与术前相比均明显升高 (P <0 0 1) ,LH组血清L - 6、TNF -α和CRP水平的升高明显低于AH组 (P <0 0 1)。LH组患者的外周血T淋巴细胞亚群和NK细胞在手术前后无明显改变 (P >0 0 5 )。AH组患者在术后 2 4hCD3+、CD4 +、CD8+均明显下降 (P <0 0 1) ,在 72h有所回升 ,但仍低于术前水平。AH组NK细胞无明显变化 (P >0 0 5 )。结论 腹腔镜手术创伤小 ,对全身免疫功能影响小。
Objective To study the effects of laparoscopic and abdominal hysterectomy on immune system. Methods A total of 78 patients with uterine myoma underwent laparoscopic hysterectomy (LH) and 42 by abdominal hysterectomy (AH) from July 2001 to July 2003.The interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α),C-reactive protein (CRP),T lymphocytes subpopulation and natural killer cell (NK) were measured 24h before and 24h,72hr after the operation. Results The concentrations of IL-6,TNF-α,CRP in both groups increased significantly after operation;and they were significantly higher in LH group than those in AH group.Postoperative levels of CD3,CD4 and CD8 in AH group were significantly lower than preoperative ones,whereas no significant changes were found in LH group. Conclusions Laparoscopic hysterectomy does not significantly affect immune function compared to AH,indicating that immunity can be preserved in the post-LH period.
出处
《中国妇产科临床杂志》
2004年第5期336-338,共3页
Chinese Journal of Clinical Obstetrics and Gynecology
关键词
腹腔镜
开腹子宫切除术
免疫功能
影响
对比研究
laparoscopic hysterectomy
interleukin-6 (IL-6)
C-reactive protein (CRP)
tumor necrosis factor-α (TNF-α)
T lymphocytes
natural killed cell
