摘要
目的 检测 2型糖尿病患者动脉粥样硬化斑块内基质金属蛋白酶 2 (MMP2 )和基质金属蛋白酶 9(MMP9)的表达和分布 ,探讨MMP2和MMP9在糖尿病动脉粥样硬化斑块中表达的作用。 方法 从 2 3例糖尿病足截肢和 17例尸检下肢动脉标本中选取晚期动脉粥样硬化病变的组织12 6块 ,糖尿病组 (74块 ) ,非糖尿病组 (5 2块 ) ,从中随机选取各 4 0块进行MMP2和MMP9抗原抗体的免疫组织化学染色 ,观察阳性物质在两组动脉粥样硬化斑块中的分布特点 ,利用计算机图像分析系统对染色程度作相对定量分析。 结果 抗MMP2、抗MMP9免疫沉积物主要集中在斑块核心周围 ,特别是在斑块的肩部和纤维帽。糖尿病组动脉内膜抗MMP2免疫沉积物表达显著高于非糖尿病组〔免疫沉淀物积分吸光度值 (IA) 6 90 14± 14 4 5 9和 5 70 0 4± 16 171,阳性面积百分比 (12 97±2 6 7) %和 (11 0 8± 3 32 ) % ,P <0 0 5〕 ;糖尿病组斑块内MMP9表达也显著高于非糖尿病组〔IA10 2 4 85± 2 0 4 31和 75 2 80± 1310 6 ,阳性面积百分比 (18 35± 3 5 9) %和 (13 6 5± 2 2 9) % ,P <0 0 1〕。 结论 MMP2和MMP9在糖尿病组动脉粥样硬化斑块中的表达显著高于非糖尿病组 ,MMP2和MMP9在糖尿病患者动脉中表达增强可以部分解释糖尿病患者易于发生动脉粥样?
Objective To investigate the potential role of matrix metalloproteinases 2(MMP-2)and MMP-9 expression in atherosclerotic plaques of diabetic patients. Methods Arterial specimens were obtained from 23 diabetic patients and from 17 autopsy cases with acute coronary syndromes. A total of 126 tissue blocks of late-staged lesions were classified into two groups: diabetic group(n =74)and non-diabetic group(n =52). Forty blocks were selected randomly from each group and serial sections were stained immunohistochemically with anti-human MMP-2(9)monoclonal antibody. Microscope Image Analyzer was used for relatively quantitative analysis. Results In the diabetic arteries,intense staining was shown around the plaque core,especially in the plaque shoulder and the thin fibrous cap. MMP2 integrated optical density( IA )of diabetic group was strikingly higher than that of the non-diabetic group. Threshold area of MMP2 was significantly elevated in samples from diabetic group compared with samples from non-diabetic group with atherosclerosis( P <0.05). IA of MMP9 was also significantly higher in diabetic group than in the non-diabetic group( P <0.01). The expression of MMP2 and MMP9 in atherosclerotic plaques of diabetic group is significantly higher than that of non-diabetic group. Conclusions The enhanced expression of MMP2 and MMP9 in the atherosclerotic plaque,which play an important role in instabilizing plaques,may promote the plaque disruption in diabetic patients.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2004年第9期612-614,共3页
Chinese Journal of Geriatrics
