摘要
目的 :探讨光氧化反应处理后的牛颈静脉带瓣管道的外观形态、组织稳定性及流体物理性能的特征。方法 :取新鲜牛颈静脉带瓣管道 2 4根 ,分为光氧化处理组、戊二醛处理组及新鲜对照组。分别观察和测量各组处理前后的管壁的厚度、管腔外径及各组管道瓣膜的抗返流功能的变化 ,测定处理前后的组织含水量、热皱缩温度、最大应力 ,并作各组的组织蛋白提取分析以了解组织稳定性。结果 :光氧化组与对照组相比管壁厚度、管腔外径、瓣膜抗返流功能、组织含水量及热皱缩温度差异无统计学意义 (P >0 .0 5 ) ,与戊二醛组相比差异有统计学意义 (P <0 .0 5 )。光氧化组最大应力大于对照组 ,低于戊二醛组。光氧化组和戊二醛组的组织蛋白提取量均少于对照组。结论 :光氧化方法处理后的牛颈静脉带瓣管道能较好地保持原有的管壁及瓣膜结构的完整性 ,并可提高组织的稳定性和组织的断裂强度。
Objective To determine the morphologic and physiochemical properties of bovine jugular conduit with valves stabilized by dye mediated photo oxidation.Methods Twenty four bovine jugular conduits with valves were divided into 3 groups and treated with dye mediated photo oxidation( Group Ⅰ),glutaraldehyde( Group Ⅱ) and untreated group(Group Ⅲ), respectively. Morphologic and physiochemical properties of the 3 groups, including wall thickness, diameter, tissue water content, heat shrinking temperature, breaching strength,and tissue protein extraction assay were studied. Results There was no difference in wall thickness, diameter, tissue water content, and heat shrinking temperature between GroupⅠand Ⅲ,but there was significant difference between Group Ⅰ and Ⅱ.The breaching strength of GroupⅠ was higher than that of Group Ⅲ (P <0.05),but lower than that of Group Ⅱ (P <0.05).A decrease in extractable tissue protein was found in GroupⅠ and Ⅱ.Conclusion The dye mediated photo oxidation can effectively preserve the structure and the anti regurgitation function of valves and improve the tissue stability and enhance the tension of bovine jugular conduit with valves.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2004年第4期429-431,共3页
Journal of Central South University ：Medical Science
基金
湖南省科技计划重大专项基金 (0 4SK1 0 0 5)
