摘要
研究了脐血造血细胞在 4℃冰箱保存过程中单核细胞活力、造血性能的变化以及细胞因子种类、组合、不同培养基添加成分对造血细胞扩增的影响。研究表明脐血在 4℃冰箱保存应不超过 3d ;细胞因子组合SCF +IL 6+FL +TPO扩增CFU C的效果最佳 ;培养基体系中添加脐血混合血浆对扩增CFU C作用明显 ,脐血混合血浆的最佳浓度为 2 5 %。
The viability of the mononuclear cells a nd the ability of producing the cell colony forming units(CFU-C) during the pe riod of preservation in refrigerator at 4 ℃ was studied; The effects of the con centration of different cytokines on the ex vivo expansion of hematopoietic cell s were investigatedThe results indicated that umbilical cord blood could not b e preserved for 3 d; The group of cytokines:SCF+IL-6+FL+TPO could most effecti vely stimulate the expansion of CFU-C Adding mixed umbilical cord blood plasm a in the IMDM medium could remarkably increase CFU-CThe optimum concentratio n of mixed umbilical cord blood plasma i n the medium was 25%
出处
《生物加工过程》
CAS
CSCD
2004年第3期46-50,55,共6页
Chinese Journal of Bioprocess Engineering
关键词
脐血
造血细胞
单核细胞
细胞因子
体外扩增
umbilical cord blood
hematopoietic cells
MNC
cyto kine
ex vivo expansion
