摘要
采用密闭式脐血采血法收集了１９２份脐血，对脐血的采集量、有核细胞数、经淋巴细胞分离液分离后的单个核细胞的回收率进行了评估用含有多种生长因子的半固体培养基对脐血所含的粒单祖细胞及红系祖细胞进行了检测，并和骨髓的同类祖细胞作了比较．结果，脐血的平均可采集量为９９ｍＬ，每份脐血所含有的有核细胞数为（１３６±４８）×１０８，分离后单个核细胞的回收率为（４２３±１６４）％，脐血的粒单祖细胞及红系祖细胞的集落产率均高于骨髓，差异有显著性，（Ｐ＜００５）．脐血形成的粒单及红系集落体积也较骨髓的同类集落体积大．
Airtight-collecting method was applied in the collection of 192 umbilical cord blood samples, in which evaluated were the volume, nucleated cell number, recovery rate of mono-nucleated cells after Ficoll separation. With semisolid culture containing growth factors, granular monocyte and erythroid lineage progenitor from cord blood were tested and analyzed with those from bone marrow. The results were indicated as follows: average collection volume was 99 mL; nucleated cell number was 13.6_4.8*108 for each blood sample, recovery rate of mono-nucleated cells was (42.3±16.4%) after Ficoll separation; colony formation rate of granular monocyte and erythroid lineage progenitor was higher in umbilical cord blood than in bone marrow, with significant difference (P<0.05); and colony formation size of granular monocyte and erythroid lineage progenitor was higher in umbilical cord blood than in bone marrow, with significant difference (P<0.05). All the above suggested that umbilical cord blood contains more hemopoietic progenitors than bone marrow.
出处
《昆明医学院学报》
1998年第2期38-41,共4页
Journal of Kunming Medical College
基金
国家自然科学基金
卫生部青年科学基金
云南省应用基础研究基金
关键词
脐血
骨髓
采集
造血细胞
半固体培养
Umbilical cord blood,Bone marrow,Cord blood collection,Hemopoietic progenitor,Semisolid culture
