摘要
目的:探讨羟乙基淀粉沉淀(HES)法分离脐血单个核细胞(MNCs)的效果。方法:采集脐血31份,应用羟乙基淀粉(HES)沉淀飞和密度离心(Ficoll)法分离脐血MNCs,、纯化CD3+细胞、CD14+细胞、CD34+细胞,以台盼蓝拒染法检测细胞活力。结果:HES法MNCs回收率、CFU-GM计数高于Ficoll法(85.29±3.79 VS 47.06±4.61,t=35.67,P<0.05;67.31±11.57/l×105MNCs VS28.54±6.39/l×105MNCs,t=16.33,P<0.05);HES法分离的MNCs中CD3+、CD14+、CD34+细胞数均高于Ficoll法(t=5.76,t=2.51,t=6.67,P<0.05)。结论:HES法分离MNCs可获得较好的回收率,是人脐血干细胞理想的分离方法。
Objeetive： To investigate the separation effect of hydroxyethyl starch （HES） precipitation for nucleated cells（MNCs）. Methods： 31 copies of cord blood were collected and separated by HI3S separation and density centrifugation （Ficoll） for MNCs, the CD3＋ cells, CD14＋ cells, and CD34＋ cells, purified by immunomagnetic technique, and cell viability were detected by trypan blue staining. Results： The rate of recollection for MNCs and CFU-GM counts using HES method were higher than that using of Ficoll method （85.29＋ 3.79 VS 47.06＋ 4.61, t=35.67, P〈0.05; 67.31＋ 11.57/1×105 MNCs VS 28.54＋ 6.39/1×10 5 MNCs, t=16.33, P〈0.05）; CD3＋, CD14＋, CD34＋ cells, using HES method were higher than that of Ficoll method （t=5.76, t=-2.51, t=-6.67, P〈0.05）. Conclusion： HES method is an appropriate method for separating HSCs.
出处
《现代生物医学进展》
CAS
2014年第29期5640-5643,共4页
Progress in Modern Biomedicine
关键词
羟乙基淀粉沉淀法
脐血造血干细胞
核细胞数
Hydroxyethyl starch precipitation
Cord blood stem cells
Nucleated cells
