摘要
为观察脂蛋白脂酶活化剂NO-1886对高糖高脂猪血浆胰岛素和胰组织中微量元素铬钒含量的影响。将15头贵州小香猪随机分为3组:对照组喂基础猪饲料,糖脂组喂高脂高蔗糖饲料,NO-1886组前3个月喂高脂高蔗糖饲料,从第4个月开始在高脂高蔗糖饲料里加1％NO-1886。动物分栏喂养,每月末抽空腹静脉血测血糖、血脂、胰岛素浓度,第8月末在测量上述指标后处死动物,取胰组织,用酸消化,等离子体原子发射光谱仪检测铬钒的含量。结果发现,对照组、糖脂组和NO-1886组的血胰岛素在加NO-1886前,分别是11.4±2.7 mU/L、21.0±4.8 mU/L和21.9±6.6 mU/L,与对照组比较,后两纽升高(P<0.05);加NO-1886后,至实验结束时分别是11.4±6.2 mU/L、20.4±2.3 mU/L和15.4±1.8 mU/L。与加NO-1886前比较,对照组与糖脂组的变化差异无统计学意义,而NO-1886组降低(P<0.05)。胰岛素敏感试验发现,对照组与NO-1886组的血胰岛素变化规律基本一致,糖脂组血胰岛素含量降低(与对照组比较,第30 min时P<0.05,第90 min时P<0.01)。实验结束时,三纽动物胰组织中钒含量分别是0.8±0.8 ng/g、0.7±0.1 ng/g和0.8±0.3 ng/g;铬的含量分别是2.3±1.2 ng/g,g、1.9±0.6ng/g和2.1±0.9 ng/g。与对照组比较,糖脂组钒铬含量降低(P<0.05),NO-1886组无显著性差异(P>0.
Aim The study aims to observe how lipoprotein lipase activator NO-1886 affect the content of vanadium and chromium in pig pancreas with high fat and high sugar. Methods Guizhou minipigs were divided into three groups randomly: control group fed with basic feedstuff, sugar fat group of fed with high fat and high sugar feedstuff, and NO-1886 group fed with high fat and high sugar feedstuff in the first 3 mouths and then added 1 % NO-1886 since the 4th mouth. The pigs were fed separately. Theirs sugar, fat and insulin of blood plasma were observed. The pigs were killed to get the pancreas at the end of the experiment and the tissue were digested with acid. The content of vanadium and chromium were tested Atomic Emission Spec trometry. Resutls The sugar fat group and NO-1886 group are as the followings: before the NO-1886 was added, the blood insulin in each group is 11.4±2.7 mU/L, 21.0± 4.8 mU/L, and 21.9 ± 6.6 mU/L, and compared with the control group, the blood insulin of the later two groups rises ( P < 0.05); then adding the NO-1886, at the end of the experiment the blood insulin in each group is 11.4 ± 6.2 mU/L, 20.4±2.3 mU/L, and 15.4± 1.8 mU/L; the content of insulin has no obvious difference between control group and NO-1886 group, while there is some difference between control group and sugar fat group ( P < 0.05) . Through insulin sensitive experiment, it was found that before injection blood insulin in each group is 19.3 ± 6.5 mU/L, 11.6 ±2.9mU/L, 19.3±7.1 mU/L, 30 min after injection the data is 123.6 ± 32.9 mU/L, 71.7 ±21.7 mU/L, and 141.5 ±29.4 mU/L, and 90 min after injection the data is 45.9±5.6 mU/L, 17.9± 12.4 mU/L, and 32.9± 12.9 mU/L, and compared with the control group, the content of insulin in the sugar fat group reduced (in the 30th min P< 0.05, in the 90th min P < 0.01). At the end of experiment, the pancreas' vanadium content in each group is0.8±0.8ng/g,0.7±0.1 ng/g, and 0.8 ± 0.3 ng/g, the chromium content is 2.3 ± 1.2 ng/g, 1.9± 0.6 ng/g, and 2.1 ± 0.9 ng/g, and compa
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第3期275-278,共4页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金(30370675)
湖南省教育厅课题(JY-01c193)资助
