摘要
目的:探讨微小RNA(micro RNA,mi RNA/mi R)-149单核苷酸多态性与华东地区汉族人群类风湿性关节炎的关联性。方法:200例类风湿性关节炎患者与120例健康对照均来源于南京市第一医院风湿免疫科2010—2014年的门诊和住院患者。在获取研究对象知情同意后,收集其临床资料并抽取2 m L外周静脉血,提取基因组DNA。利用限制性片段长度多态性聚合酶链反应(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术对研究对象mi R-149 rs22928323的单核苷酸多态性进行检测。比较两组对象相关临床参数与单核苷酸多态性的相关性。结果:类风湿性关节炎患者与健康对照组mi R-149 rs22928323的3种基因型TT,TC,CC分别为25.3%,51.1%,23.6%和18.3%,20.0%,61.7%;与mi R-149基因型TT+TC相比,基因型为CC的患者发病风险增加[OR=1.38,95%CI(1.01~1.75),P=0.023]。R A患者mi R-149各基因型间类风湿因子、肌苷、抗角蛋白抗体等临床参数比较,差异无统计学意义(P>0.05)。结论:Mi R-149的单核苷酸多态性位点rs22928323与华东地区汉族人群类风湿关节炎的遗传易感性相关;其多态性与RA患者相关临床参数间没有相关性。
Objective: To investigate the association between micro RNA(mi R)-149 polymorphism and susceptibility to rheumatoid arthritis(RA), as well as the clinical characteristics in patients with RA. Methods: A total of 200 RA patients and 120 healthy controls were recruited from Department of Rheumatology and Immunology of Nanjing First Hospital. After obtaining the informed consent, we collected 2 mL of anti-coagulated venous blood samples from all studied subjects to isolate the whole blood genomic DNA, and the clinical data were collected as well. Single nucleotide polymorphisms of mi R-149 rs22928323 were detected by polymerase chain reactionrestriction fragment length polymorphism(PCR-RFLP). Correlation between single nucleotide polymorphisms and clinical features were compared. Results: The frequencies of TT, TC and CC for rs22928323 of miR-149 were 25.3%, 51.1% and 23.6% or 18.3%, 20.0% and 61.7% in the patients or the healthy controls, respectively. The onset risk of allele C in RA patients was increased compared with allele T [OR=1.38, 95% CI(1.01–1.75), P=0.023]. There were no significant difference in rheumatoid factor, blood urine nitrogen, antikeratin antibody, and other clinical characteristics among the 3 genotypes in RA patients(P>0.05). Conclusion: SNP rs22928323 in mi R-149 is correlated with RA in the east of Chinese Han population, whereas there is no correlation between mi R-149 polymorphism and clinical characteristics in patients with RA.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2015年第5期495-498,共4页
Journal of Central South University ：Medical Science
