摘要
以稻草粉和麸皮为主要原料,对白腐菌(White-rot fungi)NS75、黑曲霉(Aspergillus niger)NS83和絮凝酵母(Saccharomyces cerevisiae)SP5混合菌固态发酵产纤维素酶进行研究。实验结果显示,在白腐菌和黑曲霉双菌混合培养2d后接入絮凝酵母,培养到第7d产酶达到峰值;三菌混合发酵产纤维素酶酶活明显高于白腐菌和黑曲霉双菌混合培养,其β-葡萄糖苷酶(β-G)和羧甲基纤维素酶(CMCase)酶活比白腐菌(White-rot fungi)NS75和黑曲霉(Aspergillus niger)NS83双菌发酵产酶分别提高了143.3%和68.2%。单因素实验和正交实验结果表明,当稻草粉麸皮质量比为8∶2,料水比为1∶2,白腐菌NS75、黑曲霉NS83和絮凝酵母SP5的接种比例为1∶2∶1.5(v/v/v)时,于30℃培养7d,固态发酵基中β-G和CMCase酶活分别达到62305U/g和30241U/g。
In the solid culture media consisted of rice straw powder and wheat bran,the cellulases production by solid state fermentation with White rot fungi NS75,Aspergillus Niger NS83 and Saccharomyces cerevisiae SP5 three strains mixing have been investigated.The results showed that the activities of cellulases produced reached the peak value at the seventh day of fermentation process,in which the yeast Saccharomyces cerevisiae SP5 was inoculated while the White rot fungi NS75 and Aspergillus Niger NS83 had grown 2 days in the solid culture media.The β-glucosidase(β-G) and carboxymethyl cellulase(CMCase) activities produced by three strains mixed fermentation have increased 143.3% and 68.2%,respectively,than that produced by two strains mixed fermentation.The single factor and orthogonal experiment results revealed that,under the optimum fermentation conditions: dosage ratio of rice straw powder and bran powder 8∶2,feed-water ratio 1∶2,inoculation volume ratio of White-rot fungi NS75,Aspergillus niger NS83 and Saccharomyces cerevisiae SP5 1∶2∶1.5(v/v/v),30 ℃ culture 7d,the β-G and CMCase activities produced reached 62305U/g and 30241U/g,respectively.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2013年第7期57-63,共7页
China Biotechnology
