摘要
用椰毒假单胞菌种特异性Ｏ－Ⅰ因子及型特异性Ｏ－Ⅳ因子的单克隆抗体Ｐｃｏ６５和Ｐｃｏ１３对从中毒银耳、正常玉米面及动物饲料中新分离的９３株椰毒假单胞菌的菌体抗原型进行了验证。ＥＬＩＳＡ判定结果表明，除１株经再次生化验证鉴定为非椰毒假单胞菌呈阴性反应外，９２株菌均含有种特异性Ｏ－Ⅰ因子，阳性符合率为１００％，其中，４７株菌含有Ｏ－Ⅳ型特异因子，占总菌数的５１．０９％，比血清学分型率（４０．２３％）高出１０．８７％。研究结果表明，鉴定椰毒假单胞菌Ｏ抗原型时、联合应用种间和型间特异性单克隆抗体进行ＥＬＩＳＡ检测，可同时定种分型，方法灵敏、快速，重复性良好。
he somatic antigens of 93 new straims of Pseudomonas
cocovenenans subsp.farinofementans isolated from poisoning tremella,
corn flour and feed were verified by monoclonal antibodis Pco 65 and
Pco 131 Which against O-1 species antigen factor and O-O-Ⅳ type
antigen factor seperately. ELISA test results showed that 92 strains
had O-1factor except one strain which was not belong to the species
of Pseudomonas cocovene-nans in later tests. So the conformabl rate
was 100 precent. 47 out of them had O -Ⅳ factor. The serotyping
rate(40.2 percent ) by McAb was 10.87 percent highr than by factor
serium,It was indicated that species and type of Pseudomonas
cocovenenans strains could be test and verify simultaneously when
both of O-monoclonal antibodies were used together, and it was
sensitive, rapid and good repeated.Author's address Liu Xiumei,
Institute of Nutrition and Food Hygiene, Chinese Academy of
Preventive Medicine, Beijing 100050,PRC
出处
《中国食品卫生杂志》
1995年第2期21-23,63,共4页
Chinese Journal of Food Hygiene
基金
卫生部科研基金资助课题
关键词
无性系鉴定
椰毒假单胞菌酵米面亚种
clonal verification
Pseudomonas cocovenenans subsp. farinofermentans
