摘要
Obejctive To investigate the cellular immune mechanism of thyroid associated ophthalmopathy (TAO) and provide a basis for treating TAO with cytokine or anti cytokine agents, we determined whether interferon (IFN) γ and interleukin (IL) 4, representative cytokines of Th1 and Th2 cells, may have some effect on the development and progression of TAO Method Retroorbital fibroblasts (RF) proliferation and the synthesis of hyaluronic acid (HA) and type Ⅳ collagen were measured with liquid scintillation and radioimmunoassay Results IFN γ stimulated RF proliferation and HA synthesis and had a significant inhibitory effect on type Ⅳ collagen synthesis IL 4 stimulated proliferation and type Ⅳ collagen synthesis in RF and had inhibitory effect on HA synthesis When IFN γ (100?U/ml) and IL 4 (1?μg/L) were incubated together with RF, they antagonized their respective stimulatory or inhibitory effect on the proliferation and synthesis of HA and type Ⅳ collagen Thyrotropin (TSH) stimulated RF proliferation and type Ⅳ collagen synthesis in a dose dependent manner, while only at high dose (100?U/L and 200?U/L), stimulated RF to synthesize HA Conclusions IFN γ, a representative cytokine of Th1 cells, is responsible for the inflammatory process of TAO; whereas IL 4, a representative cytokine of Th2 cells, has some effect on the repair process IL 4 could antagonize the inflammatory effect of IFN γ on RF TSH may have aggravating effect on the pathogenesis of TAO
Obejctive To investigate the cellular immune mechanism of thyroid associated ophthalmopathy (TAO) and provide a basis for treating TAO with cytokine or anti cytokine agents, we determined whether interferon (IFN) γ and interleukin (IL) 4, representative cytokines of Th1 and Th2 cells, may have some effect on the development and progression of TAO Method Retroorbital fibroblasts (RF) proliferation and the synthesis of hyaluronic acid (HA) and type Ⅳ collagen were measured with liquid scintillation and radioimmunoassay Results IFN γ stimulated RF proliferation and HA synthesis and had a significant inhibitory effect on type Ⅳ collagen synthesis IL 4 stimulated proliferation and type Ⅳ collagen synthesis in RF and had inhibitory effect on HA synthesis When IFN γ (100?U/ml) and IL 4 (1?μg/L) were incubated together with RF, they antagonized their respective stimulatory or inhibitory effect on the proliferation and synthesis of HA and type Ⅳ collagen Thyrotropin (TSH) stimulated RF proliferation and type Ⅳ collagen synthesis in a dose dependent manner, while only at high dose (100?U/L and 200?U/L), stimulated RF to synthesize HA Conclusions IFN γ, a representative cytokine of Th1 cells, is responsible for the inflammatory process of TAO; whereas IL 4, a representative cytokine of Th2 cells, has some effect on the repair process IL 4 could antagonize the inflammatory effect of IFN γ on RF TSH may have aggravating effect on the pathogenesis of TAO
基金
ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina (No 39470 341)
