摘要
本工作将M-胆碱能受体阻断剂东莨菪碱,r-氨基丁酸及其受体阻断剂印防己毒素,经埋植的慢性导管注入家兔双侧海马CA_3区,观察上述药物对已巩固的操作式食物性条件反应的影响。发现东莨菪碱(每侧45微克/5微升)、r-氨基丁酸(每侧3毫克/5微升)均使条件反应抑制;若注r-氨基丁酸之前先加注印防己毒素,则抑制时间缩短,且剂量愈大时间更为缩短。抑制期间动物的摄食和运动功能正常,抑制后条件反应皆恢复;注生理盐水作自身对照则均无抑制效应。这表明海马CA_3区在长时记忆的保持上起着重要作用,且此种作用有胆碱能突触和GABA能突触参与。
Scopolamine (M-cholinergic receptor antagonist), GABA and Picrotoxin(GABA receptor antagonist) were injected into the hippocampal area CA_3 ofrabbits with chronically embedded cannula. Rabbits were trained to performthe consolidatory operant conditioning. The influences of such injectionon the conditioned response were investigated. Injection of Scopolamine (45 ug/ul) caused a temporary depressionin the conditioned response with a time course of 5.4±0.5 min. (M±SE,n=10), while the animals' general motor activities and food intakewere not significantly affected. Application of GABA (3 mg/ul) alsocaused suppression for 58.2±5.6 min. (n=9). However, after a prior inje-ction of Picrotoxin (0.2 ug or 0.5 ug/ul) the depression of the conditionedresponse by application of GABA was relevantly reduced, in which casethe time course of depression was 38.7±4.6 min. (n=5, P 0.001) or 13.7±3.8 min. (n=6, P 0.001). As a control, when aline was injected into thesame loci no detectable effect on the conditioned response was observed. It seems that M-cholinergic receptor and GABA receptor in hipp-ocampal areaCA_3 are involved in the retention of long-term memory.
出处
《心理学报》
1988年第1期52-57,共6页
Acta Psychologica Sinica
