摘要
目的:探索口腔扁平苔藓(Oral Lichen Panus,OLP)的中医证候研究方法,探寻阴虚火旺型OLP的miRNAs表达特征,分析实验获得的阴虚火旺型OLP差异表达miRNAs的靶基因信号通路。方法:选取3例阴虚火旺型口腔扁平苔藓患者及3例正常人血液,分离提取miRNA,荧光标记后与miRNA基因芯片杂交,SAM软件筛选阴虚火旺型OLP患者和正常人有表达差异的miRNA,运用Targetscan软件及Kegg和Biocarta数据库分析阴虚火旺型OLP差异表达miRNAs靶基因信号通路。结果:获得5个口腔扁平苔藓特异表达miRNA,3个下调,2个上调,其中hsa-miR-18a miRNA相关的有统计学意义的信号通路共12条(P<0.01);hsa-miR-99bmiRNA基因相关的有统计学意义的信号通路有2条(P<0.05)。结论:基因芯片技术可用于口腔扁平苔藓中医证候研究,hsa-miR-18a上调和hsa-miR-99b下调可能是阴虚火旺型口腔扁平苔藓的标志性mi RNA,并且hsa-miR-18a可能通过12条信号通路调控OLP的发生发展,以及hsa-miR-99b可能通过2条信号通路调控OLP的发生发展。
Objective:To study the method of researching TCM syndrome of oral lichen panus(OLP),and to identify the specific miRNAs associated with OLP of hyperactivity fire due to yin deficiency type,and to analyze signal transduction pathway of differentially expressed miRNAs.Method:The miRNAs were extracted and isolated from six samples of blood,including 3 cases with OLP of hyperactivity fire due to yin deficiency type and 3 cases of the control group.miRNAs microarrays were used for screening the expression differences of miRNAs.The signal transduction pathway were analyzed using Targetscan software,Kegg and Biocarta databases.Result:A total of 5 differentially expressed miRNAs were found to be associated with OLP.3 miRNAs were down-regulated and 2 miRNAs were up-regulated.Among them,hsa-miR18a was corresponding to 12 pathways(P<0.01),and hsa-miR-99b was corresponding to 2 pathways(P<0.05).Conclusion:Gene chip can be used for the research on TCM syndrome of OLP.Hsa-miR-18a and has-miR-99b may be act as the key regulator of gene expression in OLP and correlate with patho genesis of OLP.And the mechanism about the regulative effect of hsa-miR18a and hsa-miR99b may be related to the adjustment of corresponding pathways.
出处
《中药与临床》
2011年第3期52-55,共4页
Pharmacy and Clinics of Chinese Materia Medica
基金
四川省科技厅科技支撑计划项目(编号:2008SZ0245)
成都中医药大学校基金重点项目(编号:ZRZD200807)
成都中医药大学附属医院科技发展基金项目(编号:2009-D-YY-09)
四川省中医药管理局临床诊疗方案研究科研项目(编号:CKY2010002
合同号:2010-14)
关键词
OLP
MIRNA
阴虚火旺
中医证候
信号通路
Oral Lichen Panus
miRNAs array
hyperactivity fire due to yin deficiency type
TCM Syndrome
signal transduction pathway
