摘要
目的研究雌激素受体α(ERα)基因及启动子超甲基化与食管鳞癌的关系。方法用RT-PCR法检测2个食管鳞癌细胞系ERαmRNA表达情况,甲基化特异PCR技术(MSP)检测ERα基因启动子超甲基化,对超甲基化的细胞用脱氧胞苷(5-aza-dC)去甲基化后检测细胞ERαmRNA。MSP检测47份组织ERα基因启动子超甲基化。结果食管鳞癌细胞系存在ERα基因启动子甲基化及其引起的ERαmRNA表达缺失。57．4％(27／47)的原发性食管鳞癌细胞有ERα基因启动子区的超甲基化,其中女性患者ERα启动子超甲基化检出率73．7％(14／19),明显高于男性(15／28,53．6％,P<0．05)。结论ERα基因启动子超甲基化与食管鳞状上皮细胞癌相关。
Objective To find the relationship between estrogen receptor alpha ( ERα) gene promoter hypermethylation caused ERαgene silencing and esophageal squamous cell carcinoma. Methods ERαexpression in 2 cell lines derived from human esophageal squamous cell carcinoma (ESCC) was tested by RT-PCR, methylated CpG was detected by methylation specific PCR ( MSP) and confirmed by in vitro de-methylation treatment with 5-aza-dC and thereafter ERαgene re-expression. ERαpromoter hypermethylation was detected by MSP in 47 ...
出处
《国际遗传学杂志》
CAS
2006年第6期401-403,433,共4页
International Journal of Genetics
基金
国家自然科学基金主任基金(No.30540081)
关键词
食管癌
甲基化
雌激素受体Α
Esophageal carcinoma
Methylation
Estrogen receptor alpha
