摘要
目的:研究食管鳞癌组织中多基因甲基化状态及其与临床病理特征的相关性。方法:提取76例食管鳞癌患者的新鲜冷冻肿瘤组织及癌旁正常组织,用酚氯仿法抽提组织中的DNA,然后用亚硫酸氢盐进行甲基化处理,最后用Real-time MSP技术分别检测了APC、RARβ2、CDH1、p16INK4α和RASSF1A5个抑癌基因的甲基化状态,结合临床及病理资料用统计软件SPSS 17.0进行统计学分析。结果:食管癌组织中5个基因DNA甲基化率显著高于癌旁正常组织(P=0.000)。肿瘤组织中5个基因DNA甲基化率与临床病理分期、淋巴结转移及神经脉管浸润均显著相关,P<0.050,且APC、p16INK4α和RASSF1A甲基化与肿瘤T分期显著相关,P<0.050,RARβ2及CDH1基因甲基化与肿瘤T分期无显著相关性(P值分别为0.320,0.105)。这5个抑癌基因甲基化与年龄、性别、肿瘤位置、肿瘤长度、分化程度、吸烟和饮酒等7项指标无显著相关性。经Logistic分析,家族肿瘤史是肿瘤组织APC基因DNA甲基化的独立相关因素〔P=0.036,Exp(B)=34.675,95%CI(1.253～959.844)〕。结论:食管癌患者肿瘤组织APC、RARβ2、CDH1、p16INK4α和RASSF1A均存在高甲基化现象,肿瘤组织中DNA甲基化与肿瘤侵袭程度显著相关;家族肿瘤史是肿瘤组织APC基因DNA甲基化的独立相关因素。
OBJECTIVE：To study the methylation status of multiple genes and the correlation between clinical pathological features and DNA methylation in tumor tissue in patients with ESCC were involved.METHODS：Seventy-six patients with ESCC.Fresh-frozen tumor specimens and adjacent normal specimens of them were collected,and DNA was extracted with phenol and chloroform.Then,aberrant promoter hypermethylation of APC,RAR β 2,CDH1,p16INK4αand RASSF1A was confirmed using MSP（methylation-specific polymerase chain reaction） after bisulfite treatment.Test data,clinical data and pathological data were combined for statistical analysis with SPSS 17.0.RESULTS：The methylation rates of the five genes in esophageal tumor tissues were significantly higher than those of adjacent normal tissues（P= 0.000）.DNA methylation rates of the five genes were significantly related to clinical pathological stage,lymph node metastasis and nerve vessel invasion（P〈0.050）.The methylation rates of APC,p16INK4α,RASSF1A were significantly correlated with tumor T stage（P〈0.050）,while those of RAR β2 and CDH1were not significantly correlated with T stage（Pvalues were 0.320and 0.105 respectively）.The methylation rates of the five tumor suppressor genes were not significantly correlated with age,sex,tumor location,tumor length,degree of differentiation,smoking and drinking.Family history of cancer was an independent related factor for the methylation of APC through Logistic analysis 〔 P=0.036,Exp（B） =34.675,95%CI（1.253-959.844）〕.CONCLUSIONS：APC,RAR β2,CDH1,p16INK4αand RASSF1Ain tumor tissues for patients with ESCC are hypermethylation and the DNA methylation rates are significantly related to the tumor invasive degree.Family history of cancer is an independent related factor for the methylation of APC.
出处
《中华肿瘤防治杂志》
CAS
2011年第23期1851-1854,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
浙江省科技计划资助项目(2009C33143)
浙江省自然基金项目(Y2080749
Y2091110)
人事部优秀留学回国人员科技活动项目(2008A004)
教育部"优秀留学回国人员科研基金"项目(2010609)
关键词
癌
鳞状细胞
食管肿瘤/病理学
甲基化
DNA
carcinoma
squmous cell
esophageal neoplasms/pathology
methylation
DNA
