摘要
目的观察细胞周期蛋白E2在下咽癌组织中的表达水平及其与临床病理特征及预后的相互关系。方法免疫组化法检测50例下咽癌肿瘤组织及相应的正常黏膜组织中细胞周期蛋白E2的表达水平,分析其与临床特征及生存率的相互关系,结果采用SPSS 18.0软件进行统计分析。结果细胞周期蛋白E2在下咽癌组织中的表达较正常黏膜组织明显增加(Z=-5.764,P=0.000),且其表达程度与肿瘤的临床分期(Z=-1.995,P=0.046)、淋巴结转移(Z=-2.176,P=0.030)相关。肿瘤组织中细胞周期蛋白E2表达阳性患者5年生存率为40.2%,阴性表达患者5年生存率为81.8%。细胞周期蛋白E2表达水平高的患者生存率明显降低(χ2=6.317,P=0.012)。结论细胞周期蛋白E2在下咽癌肿瘤组织中的异常表达提示其可能参与了肿瘤的发生发展,并有可能成为下咽癌的诊断、治疗与预后的分子标志物之一。
Objective This study aims to evaluate the level of expression of cyclin E2 in hypopharyngeal cancer and its relationship with clinical characteristics as well as prognosis.Methods The protein levels of cyclin E2 in tumor tissues and adjacent normal tissues were assessed by immunohistochemistry staining in 50 cases of hypopharyngeal cancer.The relationship between the expression level of cyclin E2 and clinical data was analyzed using the SPSS software.Results Protein levels of cyclin E2 in hypopharyngeal cancer were higher than protein levels of cyclin E2 in normal tissue(Z=-5.764,P=0.000).The cyclin E2 level in tumor tissue correlated with the tumor staging(Z=-1.995,P=0.046)and lymph node metastasis(Z=-2.176,P=0.030).The 5-year survival rates of patients with cyclin E2 positive and negative expression were 40.2%and 81.8%,respectively.Patients with high expression levels of cyclin E2 had a lower survival rate(χ~2=6.317,P=0.012).Conclusion The abnormal expression of cyclin E2 protein in tumor tissues indicates that cyclin E2 may be involved in the development of hypopharyngeal cancer and could be a potential diagnostic and prognostic molecular marker.
作者
黄俊伟
陈学军
刘宏飞
陶冶
李祖飞
陈平
张洋
HUANG Junwei;CHEN Xuejun;LIU Hongfei;TAO Ye;LI Zufei;CHEN Ping;ZHANG Yang(Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Key Laboratory of Otolaryngology Head and Neck Surgery,Ministry of Education,Beijing 100730,China)
出处
《山东大学耳鼻喉眼学报》
CAS
2020年第5期132-137,共6页
Journal of Otolaryngology and Ophthalmology of Shandong University
基金
首都卫生发展科研专项(2018-1-2052)
国家自然科学基金(81670946)
关键词
细胞周期蛋白E2
下咽癌
数据库检索
免疫组化
生存分析
预后因子
Cyclin E2
Hypopharyngeal carcinoma
Database retrieval
Immunohistochemistry
Survival analysis
Prognosis factor
