摘要
目的探究巴豆油对寒积便秘大鼠的治疗作用及毒性,阐明巴豆油治疗寒积便秘的“毒-效”关系。方法大鼠连续3天灌胃2℃冰水的10%活性炭溶液复制寒积便秘模型,于第4天灌胃巴豆油,观察记录大鼠首次排便时间和3 h内排便次数;HE染色评价大鼠结肠组织一般病理情况;检测大鼠血清中白介素(Interleukin,IL)-6、IL-10、肿瘤坏死因子(Tumor necrosis factor,TNF)-α、环氧合酶(Cyclooxygenase,COX)-2、巨噬细胞炎性蛋白(Macrophage inflammatory protein 1,MIP-1)、谷丙转氨酶(Alanine aminotransferase,ALT)、谷草转氨酶(Aspartate aminotransferase,AST)及总蛋白(Total protein,TP)水平;免疫组化检测大鼠结肠组织中髓样分化因子(Myeloid differentiation factor 88,MyD88)和核转录因子(Nuclear transcription factor,NF)-κB p65的表达。结果巴豆油在各剂量下均能不同程度地缩短寒积便秘大鼠首次排便时间并增加3 h内排便次数,其中3.78、2.83和1.89 g·kg^(-1)巴豆油组具有显著统计学意义(P<0.05);HE染色结果显示,与模型组相比,巴豆油在各剂量下均能导致大鼠结肠组织黏膜出现不同程度的炎症及灶性坏死,杯状细胞缺失和固有层水肿,且以3.78 g·kg^(-1)巴豆油组大鼠结肠组织损伤最为严重;此外,与模型组相比,巴豆油各组大鼠血清中COX-2、TNF-α、IL-6水平均有不同程度的提高(P<0.05),且以3.78 g·kg^(-1)巴豆油组效果最为明显;巴豆油在各剂量下均能使大鼠血清中MIP-1水平上升,且3.78 g·kg^(-1)巴豆油组效果明显(P<0.01);但巴豆油各组大鼠血清中IL-10水平的变化不明显;免疫组化结果显示,除0.47 g·kg^(-1)剂量组外,巴豆油各剂量均能造成大鼠结肠组织中MyD88蛋白水平表达增加,其中3.78、2.83和1.89 g·kg^(-1)剂量组差异具有显著性(P<0.05);巴豆油各剂量均能增加大鼠结肠组织中NF-κB p65蛋白水平,其中3.78 g·kg^(-1)巴豆油组差异具有显著性(P<0.05)。此外,与模型组相比,巴豆�
Objective To explore the therapeutic effect and toxicity of Croton tiglium L.(croton)oil on rats with cold accumulation constipation,and to clarify the toxic-effect relationship of croton oil in the treatment of cold accumulation constipation.Methods The rats were orally treated with 10%2℃activated carbon solution for 3 consecutive days to replicate the model of cold constipation.The rats were fed with croton oil on the fourth day,and then the time of first defecation and the number of defecations within 3 h were observed and recorded.HE staining was used to evaluate the general pathological conditions of the colon tissues of rats.The serum levels of interleukin(IL)-6,IL-10,tumor necrosis factor(TNF)-α,cyclooxygenase(COX)-2,macrophage inflammatory protein(MIP)-1,alanine aminotransferase(ALT),aspartate aminotransferase(AST),and total protein(TP)were detected.Immunohistochemistry was used to detect the expression of myeloid differentiation factor 88(MyD88)and nuclear transcription factor(NF)-κB P65 in colon tissues of rats.Results Croton oil could shorten the time of first defecation and increase the number of defecations within 3h in rats with cold accumulation constipation to varying degrees,and the 3.78,2.83,and 1.89 g·kg^(-1) croton oil group had significant statistical significance(P<0.05).HE staining results showed that compared with the model group,croton oil group could cause different degrees of inflammation and focal necrosis,goblet cell loss and lamina propria edema in the colon tissue of rats,and the 3.78 g·kg^(-1) croton oil group was the most serious in the colon tissue injury.In addition,compared with model group,croton oil group could increase the levels of COX-2,TNF-α and IL-6 in serum to varying degrees(P<0.05),and 3.78 g·kg^(-1) croton oil group had the most obvious effect.The level of MIP-1 in serum was increased in croton oil group,and the effect was significant in 3.78 g·kg^(-1) croton oil group(P<0.01).But croton oil group had no significant effect on serum IL-10 level of rats.The im
作者
陈俊仁
王代波
李轶
彭成
熊亮
谢晓芳
曹小玉
Chen Junren;Wang Daibo;Li Yi;Peng Cheng;Xiong Liang;Xie Xiaofang;Cao Xiaoyu(Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第9期3164-3173,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
四川省科学技厅重点研发项目(2018JZ0081):西南特色毒性中药增效解毒机制研究,负责人:曹小玉
国家中医药管理局国家中医药多学科交叉创新团队项目(ZYYCXTD-D-202209):西南特色中药资源多维评价多学科交叉创新团队,负责人:彭成。
关键词
巴豆油
寒积便秘
大鼠
“毒-效”关系
炎性因子
Croton tiglium L.oil
Cold accumulation constipation
Rats
Toxic-effect relationship
Inflammatory cytokines
