摘要
目的:研究附子配伍甘草前后附子生物碱类成分和甘草主要成分在大鼠不同肠段(十二指肠、空肠、回肠)的吸收特征,从而探讨附子-甘草配伍前后在体肠吸收的差异。方法:采用大鼠在体肠灌注模型,以紫外分光光度法测定酚红质量浓度,利用液相色谱-质谱联用法测定灌注液中乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、苯甲酰新乌头原碱、甘草酸、甘草次酸、甘草苷的质量浓度,计算肠吸收动力学参数。结果:首先比较附子-甘草合煎液中9个成分在三个肠段中的吸收差异,结果显示附子-甘草合煎液中乌头碱在三个肠段吸收差异性不大;次乌头碱在回肠吸收速度最快,与十二指肠和空肠比有显著差异(P<0.05);新乌头碱在十二指肠中吸收最慢,和空肠比有显著差异(P<0.05)。单酯型生物碱在回肠吸收速度最快,与十二指肠和空肠比都有显著差异(P<0.05),其中苯甲酰新乌头原碱在十二指肠吸收和空肠比有显著差异(P<0.05)。甘草酸在十二指肠吸收速度显著大于空肠和回肠,甘草次酸在十二指肠吸收速率显著大于空肠(P<0.05)。比较附子-甘草配伍前后各成分在同一肠段中吸收差异,结果表明附子-甘草合煎液中乌头碱和新乌头碱在十二指肠、空肠及回肠的吸收速率都有所降低,合煎液中次乌头碱在回肠的吸收速率大于单煎液,但无显著性差异,在十二指肠和空肠吸收速率降低(P<0.05);与甘草单煎液比较,合煎液甘草中的甘草酸和甘草次酸在十二指肠和空肠中的吸收速率明显升高,而甘草苷在空肠和回肠吸收速率明显升高(P<0.05)。结论:"附子-甘草"药对中甘草的主要有效成分首先在十二指肠吸收,附子中的主要毒/效成分在回肠吸收,同时附子配伍甘草后乌头碱吸收缓慢,起到一定缓释减毒的作用,此为甘草减附子之毒的重要机制。
Objective:To investigate the absorption characteristics of alkaloids in Fuzi and main ingredients in Gancao in different intestinal segments(duodenum,jejunum,and ileum)and explore the difference in intestinal absorption before and after Fuzi-Gancao compatibility.Methods:The rat model of intestinal perfusion in vivo was employed in this study.The concentration of phenol red was determined by ultraviolet spectrophotometry.The mass concentrations of aconitine,hypaconitine,mesaconitine,glycyrrhizic acid,glycyrrhetinic acid,liquiritin,benzoylaconine,benzoylhypaconine,and benzoylmesaconine were determined by the liquid chromatography-mass spectrometry(LC-MS),and the kinetic parameters of intestinal absorption were calculated.Results:As revealed by the comparison of the difference in absorption of nine ingredients in Fuzi-Gancao decoction in three intestinal segments,no significant difference was observed in the absorption of aconitine of Fuzi-Gancao decoction in the three intestinal segments.The absorption rate of hypaconitine in the ileum was the highest,and significant differences were observed as compared with those in the duodenum and the jejunum(P<0.05).The absorption rate of mesaconitine in the duodenum was the lowest,with significant difference as compared with that in the jejunum(P<0.05).The absorption rate of monoester alkaloids in the ileum was the highest,with significant difference as compared with those in the duodenum and the jejunum(P<0.05).The absorption rate of benzoylmesaconine showed significant difference in the duodenum and the jejunum(P<0.05).The absorption rate of glycyrrhizic acid in the duodenum was significantly higher than those in the jejunum and the ileum.The absorption rate of glycyrrhetinic acid in the duodenum was significantly higher than that in the jejunum(P<0.05).As displayed by the comparison of the difference in absorption of each ingredient in Fuzi and Gancao before and after their compatibility in the same intestinal segment,the absorption rates of aconitine and mesaconitine in F
作者
李文
曾佑琴
周春燕
张斌
刘德明
Li Wen;Zeng Youqin;Zhou Chunyan;Zhang Bin;Liu Deming(State Key Laboratory of Southwest Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137;School of Medical Technology,Chengdu University of Traditional Chinese Medicine,Chengdu 611137;Chongqing Traditional Chinese Medicine Hospital,Chongqing 400011)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2021年第6期106-111,共6页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金青年项目(编号:81903812)
重庆市自然科学基金(编号:cstc2020jcyj-msxmX1003,cstc2018jcyjAX0370)
关键词
附子
甘草
在体肠吸收
减毒机制
Fuzi
Gancao
in situ intestinal absorption
mechanism of reducing toxicity
