摘要
目的:检测鼻咽癌中微小RNA-221-3p(miR-221-3p)的表达,分析其与增殖、凋亡指标的相关性。方法:选取鼻咽癌患者69例作为观察组,慢性鼻炎患者69例作为对照组。均留取组织标本,应用实时荧光定量聚合酶链反应法检测两组中miR-221-3p的表达。蛋白免疫印迹法检测鼻咽癌中BCL-2相关X蛋白(BAX)的表达。免疫组织化学法检测鼻咽癌中Ki67的表达。原位杂交法检测鼻咽癌中EB病毒的表达。结果:观察组miR-221-3p表达高于对照组,差异有统计学意义(P<0.05),miR-221-3p的表达在观察组不同组织学亚型、淋巴结转移、局部浸润程度的分组中差异有统计学意义(P<0.05)。生存分析显示miR-221-3p高表达患者的生存时间短(P<0.05)。Spearman相关分析显示,miR-221-3p表达与Ki67增殖指数呈正相关(r=0.56,P<0.05),与BAX呈负相关(r=-0.69,P<0.05)。结论:与慢性鼻炎患者比较,鼻咽癌中miR-221-3p表达升高。MiR-221-3p高表达促进肿瘤增殖、抑制凋亡,预示病变呈进展状态及较差的预后。
Objective:To detect the expression of microRNA-221-3 p(miR-221-3 p)in nasopharyngeal carcinoma,analyze its correlation with cell proliferation and apoptosis indexes.Methods:A total of 69 patients with nasopharyngeal carcinoma were selected as the observation group.69 cases of chronic rhinitis were selected as the control group.Tissue sample were retained.Expression of miR-221-3 p was detected by real-time fluorescent quantitative polymerase chain reaction(qPCR)in two groups.Expression of BCL2 associated X protein(BAX)was detected by Western blotting in observation group.Expression of Ki67 was detected by immunohistochemistry in observation group.Expression of EBV was detected by in situ hybridization in observation group.Results:Expression of miR-221-3 p in observation group was significantly higher than that in control group(P<0.05).Expression of miR-221-3 p was significant different in histological subtypes,lymph node metastasis and local infiltration degree(all P<0.05).MiR-221-3 p was negatively related to survival time by survival analyze(P<0.05).Positive correlation was found between miR-221-3 p and Ki67 proliferation index(r=0.56,P<0.05).Negative correlation was found between miR-221-3 p and BAX(r=-0.69,P<0.05).Conclusion:Compared with the control group,expression of miR-221-3 p was higher in observation group.The high expression of miR-221-3 p promotes tumor proliferation and inhibits apoptosis,which indicates the poor biological behavior and prognosis.
作者
戴润芝
彭先兵
DAI Runzhi;PENG Xianbing(Department of Otolaryngology,Affiliated People's Hospital of Hubei Medical College,Shiyan 442000,China)
出处
《现代医学》
2020年第12期1572-1576,共5页
Modern Medical Journal
