摘要
目的在流体剪切应力条件下,探究力学微环境对嗜中性粒细胞在活化血小板上滚动黏附的影响。方法实验在平行平板流动腔中进行。流动腔底板通过吸附v WF-A1而功能化,黏附于功能化底板的血小板通过灌注PBS施加1 Pa壁面剪切应力(wall shear stress,WSS)刺激,力学刺激时间分别为0、2.5和7.5 min。利用高速摄像机观察和记录WSS=50 m Pa条件下白细胞在活化血小板上的滚动黏附行为,提取黏附事件数、细胞拴缚停留时间及滚动速度等参数。结果中性粒细胞能特异性的结合到v WF-A1介导的稳定黏附于流动腔底部的血小板上;白细胞在血小板上的拴缚停留时间不依赖于力学刺激,但力学刺激会显著促进白细胞在血小板上的黏附,降低白细胞在血小板上的滚动速度。结论力学刺激血小板将显著提高血小板捕获循环白细胞的能力,促进白细胞在血小板上的滚动黏附。
Objective To investigate the effects of fluid shear stress on rolling adhesion of neutrophils on immobilized platelets under flows.Methods Experiments were performed at the parallel plate flow chamber.Platelets were adhered to the functionalized flow chamber bottom which were coated with v WF-A1 first,and then washed with PBS under wall shear stress(WSS)of 1 Pa for different time(0 min,2.5 min,7.5 min).A high-speed camera was used to observe and record the rolling adhesion events of neutrophils on immobilized platelets under 50 m Pa WSS,and the adhesion parameters such as the number of adhesion events,the tether lifetime of cells and rolling velocity.Results Neutrophils could specifically bind to the immobilized platelets on v WF-A1-coated bottom of the flow chamber.Mechanical stimulation on immobilized platelets had no effects on the tether lifetime of neutrophils on the platelets,but up-regulated the adhesive ratio of neutrophils on the platelets and slowed down the rolling of neutrophils on the platelets.Conclusions Mechanical stimulation on the immobilized platelets will significantly make the circulating neutrophils to be captured easily and promote the rolling adhesion of neutrophils on platelets.
作者
潘雨平
冯晶晶
蒋笑嫣
凌颖琛
吴建华
PAN Yuping;FENG Jingjing;JIANG Xiaoyan;LING Yingchen;WU Jianhua(Institute of Biomechanics,School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China)
出处
《医用生物力学》
EI
CAS
CSCD
北大核心
2020年第1期43-48,共6页
Journal of Medical Biomechanics
基金
国家自然科学基金项目(11432006,11672109,11702100,31170887).
