摘要
目的建立对中毒者血液中一氧化二氮快速、准确的定性定量检验方法。方法取0.5 mL血,置于10 mL顶空瓶中,加0.5 mL水稀释,加入内标二氯甲烷1μL,封口,60℃水浴加热20 min,抽取250μL上层气体进气相色谱-介质阻挡放电氦等离子体检测器(GC-BID)分析。结果一氧化二氮在血液饱和溶解度的2%~100%范围内线性关系良好,检出限为血液饱和溶解度的1%。结论本方法快速、准确、有效,可用于血中一氧化二氮的定性定量分析。
Objective To establish a rapid and accurate qualitative and quantitative testing method for nitrous oxide in the blood of poisoned patients.Methods Take 0.5 mL of blood and transfer it to a 10 mL headspace bottle.Add 0.5 mL of water for dilution and introduce 1μL of dichloromethane as the internal standard.Seal the bottle and heat it in 60℃water bath for 20 min.Extract 250μL of the upper gas and analyze it using gas chromatography-dielectric barrier discharge ionization detector(GC-BID).Results The linearity of nitrous oxide is good in the range of 2%~100%of the saturated solubility of blood,with the detection limit of 1%of the saturated solubility of blood.Conclusion The method is rapid,accurate and efficient,enabling both qualitative and quantitative analysis of nitrous oxide in blood.
作者
吕惊晗
商金磊
蒋华宇
石辉丽
郭杰
魏立青
李彭
LV Jing-han;SHANG Jin-lei;JIANG Hua-yu;SHI Hui-li;GUO Jie;WEI Li-qing;LI Peng(Technology Department of Criminal Police in Qingdao,Qingdao 266034,China;Key Laboratory of Drug Monitoring and Control,Drug Intelligence and Forensic Center,Ministry of Public Security,P.R.C.,Beijing 100193,China)
出处
《中国药物依赖性杂志》
CAS
CSCD
2023年第4期323-325,共3页
Chinese Journal of Drug Dependence
基金
十四五国家重点研发计划纳米前沿专项课题(2022YFA1205504)
