摘要
该研究选择高血压前期肝火亢盛证患者为研究对象,基于UPLC-Q-Exactive MS技术,采用非靶向代谢组学研究方法分析高血压前期肝火亢盛证患者和健康志愿者的血浆样本,并结合多元统计学及代谢通路分析方法,筛选高血压前期肝火亢盛证患者的差异代谢标志物和代谢通路。分析鉴定得到雄酮、溶血磷脂酰胆碱等33个潜在生物标志物和甾类激素、脂质代谢等16条相关代谢通路,并初步构建了中医证候的代谢组学PLS判别模型:Y=-0.070X13-0.006X8+0.040X5-0.152X1+0.131X10+0.036X11+0.043X23+0.076X16+0.132X20+0.081X19-0.101X31+0.082X15-0.038X9+0.079X24。模型预测值为88.1%,解释能力为88.4%。该研究采用代谢组学方法能区分并揭示高血压前期肝火亢盛证的特征性代谢模式,PLS判别模型的初步构建为高血压前期的中医证候辨识提供了客观的辨证依据,为从小分子层面及整体水平探究中医证候的生物学基础提供了思路与方法。
In this study, patients with prehypertensive liver-fire hyperactivity syndrome(LFHS) were selected as the research objects. The plasma samples of healthy volunteers and patients with prehypertensive LFHS were analyzed by non-targeted metabolomics based on UPLC-Q-Exactive MS. The differential biomarkers and metabolic pathways were screened out by multivariate statistics and metabolic pathway analysis, which revealed the characteristics of metabolic patterns of the syndrome. Thirty-three potential biomarkers such as androsterone and lysophosphatidylcholine and 16 related metabolic pathways such as steroid hormone metabolism and lipid metabolism were identified, and a partial least squares-discriminant analysis(PLS-DA) model of traditional Chinese medicine(TCM) syndromes was preliminarily constructed: Y =-0.070X13-0.006X8+ 0.040X5-0.152X1+0.131X10+0.036X11+0.043X23+0.076X16+0.132X20+0.081X19-0.101X31+0.082X15-0.038X9+0.079X24. The predictive value of the model was 88.1%, and the explanatory power was 88.4%. In this study, the characteristic metabolic pattern of the prehypertensive LFHS was distinguished and revealed by metabolomics. The constructed PLS-DA model is expected to provide an objective basis for the identification of TCM syndromes in prehypertension, and inspiration for exploring the biological basis of TCM syndromes at small-molecular and overall levels.
作者
王宇
李淑敏
李超
杨雯晴
李运伦
WANG Yu;LI Shu-min;LI Chao;YANG Wen-qing;LI Yun-lun(Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Ji'nan 250011,China;Shandong University of Traditional Chinese Medicine,Ji'nan 250355,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第11期2881-2888,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81974555)
山东省“泰山学者”建设工程专项(ts201712042)
山东省医药卫生科技发展计划项目(202003011161)。
关键词
高血压前期
肝火亢盛证
代谢组学
prehypertension
liver-fire hyperactivity syndrome(LFHS)
metabolomics
