摘要
采用制备薄层色谱、硅胶柱色谱、ODS柱色谱、D101大孔树脂柱色谱、Sephadex LH-20凝胶柱色谱等色谱分离方法,对北豆根的75%乙醇提取物进行分离纯化,根据化合物的理化性质和波谱数据进行结构鉴定。从北豆根的75%乙醇提取物中分离得到15个化合物,分别鉴定为N-methylcorydaldine(1)、白蓬草福林(2)、千金藤啶碱(3)、阿克吐明(4)、蝙蝠葛苏林碱(5)、阿克吐米定(6)、蝙蝠葛新苛林碱(7)、蝙蝠葛辛碱(8)、6-O-去甲基蝙蝠葛波酚碱(9)、蝙蝠葛定碱(10)、蝙蝠葛糖苷(11)、刺五加苷D(12)、马兜铃内酯(13)、马兜铃酸萜酯Ⅰ(14)和马兜铃酸(15),化合物12～15为首次从蝙蝠葛属植物中分离得到。利用MTT法对所分得量较大的6种生物碱进行了抗缺氧活性研究,结果表明6种生物碱均具有较显著的抗缺氧活性。
To study the chemical constituents from the rhizome of Menispermum dauricum, fifteen compounds,N-methylcorydaldine (1), thalifoline (2), stepholidine (3), acutumine (4), daurisoline ( 5), acutumidine ( 6), dauricicoline (7), bianfugecine (8), 6-O-demethylmenisporphine (9), bianfugedine (10), dauricoside (11), eleutheroside D (12), aristolactone (13), aristoloterpenate Ⅰ(14) and aristolochic acid (15) were isolated from 75% ethanol extract of Menispermi Rhizoma by using thin layer chromatography and column chromatography methods. Their structures were identified based on their physicochemical properties and spectral data. Among them, compounds 12-15 were obtained from the genus Menispermum for the first time. Six alkaloids with higher contents were subjected to evaluate the anti-hypoxic activities by using MTT method. As a result, six alkaloids exhibited significant anti-hypoxia activities.
作者
邵佳
师超峰
魏金霞
李雅潇
郭兴杰
SHAO Jia;SHI Chao-feng;WEI Jin-xia;LI Ya-xiao;GUO Xing-jie(Department of Pharmacy, Tianjin First Central Hospital, Tianjin 300192, China;Department of Health Service, Logistics College of Chinese People's Armed Police Forces, Tianjin 300309, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第4期723-729,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81703690
81803356)
武警后勤学院博士启动金项目(WHB201711)
武警后勤学院科研基金面上项目(WHJ201717)
天津市第一中心医院科技基金项目(院CM201806)
关键词
北豆根
化学成分
生物碱
抗缺氧活性
Menispermum dauricum
chemical constituents
alkaloids
anti-hypoxia activities
