摘要
目的:建立高效液相色谱法测定丹芩颗粒中黄芩苷和丹酚酸B的含量。方法:采用Dionex高效液相色谱仪,色谱柱为Diomand C_(18)柱(200 mm×4.6 mm,5μm);测定黄芩苷的流动相为甲醇-水-磷酸(43:57:0.2),检测波长为280 nm;测定丹酚酸B的流动相为甲醇-乙腈-水-甲酸(27:8:63:2),检测波长为286 nm;流速为1.0 mL·min^(-1);柱温30℃。结果:在测定条件下,黄芩苷和丹酚酸B的浓度分别在15.0～90.2μg·mL^(-1)(r=0.9998)和48.7～207μg·mL^(-1)(r=0.9999)范围内线性关系良好;方法回收率(n=6)分别为98.3%(RSD=1.4%)和97.1%(RSD=1.2%)。结论:本方法操作简便,准确度高,专属性强,适用于丹芩颗粒的质量控制。
Objective：To establish HPLC method for determination of baicalin and salvianolic acid B in Danqin granules. Method：The HPLC method was performed with Dionex series and Diomand C18 column（200 mm × 4. 6mm,5μm）. The mobile phase for baicalin determination was methanol -water -phosphoric acid （43：57：0. 2）,with detective wavelength at 280 nm. The mobile phase for salvianolic acid B determination was methanol - acetonitrile- water- formic acid （27： 8： 63：2）, with detective wavelength at 286 nm. The flow rate was 1.0 mL · min^-1 and the column temperature was 30 ℃. Results：There were good linearity between the peak area and concentration within the ranges of 15.0 - 90. 2μg · mL^-1 for baicaLin and 48.7 - 207 μg · mL^-1 for salvianolic acid B, respectively. The average recoveries（ n =6） of baicalin and salvianolic acid B were 98.3% （RSD = 1.4% ） and 97.1%（ RSD = 1.2% ） ,respectively. Conclusion ：The HPLC method is simple, sensitive and repetitive, and is suitable for quality control of Danqin granules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第10期1738-1740,共3页
Chinese Journal of Pharmaceutical Analysis
基金
天津市科技攻关重大科技工程项目(05ZHGCGX01400)
