摘要
目的建立搜风理肺丸中黄芩苷的含量测定方法。方法高效液相色谱法(HPLC)测定搜风理肺丸中黄芩苷的含量。采用Agilent Extend—C18(250mm×4.6mm,5μm)柱,以甲醇-0.05%磷酸水溶液(42:58V/V)为流动相,检测波长为280nm。结果黄芩苷在9.82~491.0mg·L^-1范围内,浓度与峰面积线性相关性良好(r=0.9999,n=7),平均回收率为103.34%(RSD为1.61%)。结论HPLC法测定方法简便、准确,可用于搜风理肺丸的质量控制。
Objective To establish the determination method for baicalin in Soufeng Lifei Wan. Methods The content of baicalin in Soufeng Lifei Wan was determined by high-performance liquid chromatography(HPLC). We carried out the experiment with Agilent Extend-18 (250 mm × 4.6 mm,5 μm ) column and methanol-0.05% phosphoric acid water(42:58 V/V) as the mobile phase. The detective wavelength was 280 nm. Results The linear range was 9. 82 - 491.0 mg · L^ - 1 ( r = 0. 999 9, n = 7). The average recovery percent was 103.34% ( RSD = 1.61% ). Conclusion The HPLC method is simple and accurate, and can be used for the quality control of Soufeng Lifei Wan.
出处
《国际药学研究杂志》
CAS
2009年第5期373-375,共3页
Journal of International Pharmaceutical Research
关键词
高效液相色谱法
搜风理肺丸
黄芩苷
含量测定
high-performance liquid chromatography
Soufeng Lifei Wan
baicalin
determination