期刊文献+

反相高效液相色谱法分离测定他达拉非的含量 被引量:2

Determination of Tadalafil by Means of RP - HPLC
下载PDF
导出
摘要 目的建立分离测定他达拉非及其产品的反相高效液相色谱法。方法以Dionex Kromasil ODS1柱(250 mm×4.6 mm,5μm)为色谱柱,流动相为甲醇-乙腈-用三乙胺调节pH值至3.0的0.05 mol/L磷酸溶液(44:13:43),流速为1.5 mL/min,检测波长为290 nm,进样量20μL。结果他达拉非线性浓度范围为11.2-86.7μg/mL,r=0.999 9(n=6),平均回收率为100.20%,RSD为0.58%(n=6),重复进样RSD为0.38%(n=5),检出限为0.9 ng(S/N=3)。结论该法简便、快速、准确,适用于测定他达拉非及其产品的含量。 Objective To develop a RP- HPLC method for the isolation and determination of tadalafil and its products. Methods Chromato- graphic conditions included: a column of Dionex Kromasil ODS1 (250 mm ×4. 6 mm,5μm), a mobile phase was a mixture of methanol, acetonitrile, 0.05 mol/L phosphoric acid (adjust with triethylamine to a pH of 3.0)(44:13:43), the flow rate was 1.50 mL/min,the detection wavelengths was at 290 nm. Results There was a good linear relationship over the range of 11.2-86. 7 μg/mL, r =0. 999 9(n = 6).The average recovery 100.20% with RSD was 0.58% (n=6).The RSD for repetition was 0.38% (n=5).The detection limit is 0.9 ng(S/N=3). Conclusion The method was simple, rapid, accurate and can be used to assay tadalafil and its products.
作者 钱亚萍
出处 《中国药业》 CAS 2006年第15期34-35,共2页 China Pharmaceuticals
关键词 他达拉非 反相高效液相色谱法 含量测定 tadalafil RP - HPLC content determination
  • 引文网络
  • 相关文献

参考文献4

二级参考文献7

  • 1Corbin JD, Francis SH. Phammacology of Phosphodiesterase-5 inhibitors[J]. Int J Clin Pract ,2002,56(6) :453. 被引量:1
  • 2Wayne JG, Albert W, James Yu, et al. T adalafil has no detrimental effect on human spermatogenesis or reproductive hormones [ J ]. J Urol,2003 ,170( 3 ) :887. 被引量:1
  • 3Porst H. IC351(tadalafil, Cialis):update on clinical experience[J].Int J Impot Res, 2002,14(Suppl 1 ): S57. 被引量:1
  • 4Brock G. Towards optimal ED management. [J]. Can J Urol,2001,8(6): 1419. 被引量:1
  • 5Saenz TI, Fredlund P, Anglin G.Cialis(TM) (IC351) asa treatment of erectile dysfunction in diabetic men [ J]. Diabetes ,2001,50: A425. 被引量:1
  • 6Brock GB, McMahon CG, Chen KK. Efficacy and safety of tadalafil for the treatment of erectile dysfunction: results of integrated analyses[J]. J Urol, 2002 ,168 :1332 . 被引量:1
  • 7李元敏.西地那非首次遇到市场竞争对手──tadalafil[J].国外医学(药学分册),2003,30(4):253-253. 被引量:2

共引文献9

同被引文献17

引证文献2

二级引证文献8

;
使用帮助 返回顶部