摘要
采用SSR技术对含有野生二粒小麦AS846抗白粉病基因的N9659进行了分子标记研究。用高感小麦白粉病的普通小麦品种陕160与N9659杂交,F1表现高抗,F2苗期抗病和感病植株的分离比例符合3∶1,表明N9659苗期白粉病抗性由1对完全显性基因控制;采用208对小麦SSR引物对"陕160×N9659"F2抗感池分析,筛选出3个在抗感池间存在多态性引物WMS67,WMS408和WMS604;经分离群体验证,该抗病基因与小麦染色体5B上的微卫星位点Xgwm67,Xgwm408和Xgwm604连锁,遗传距离分别为6.7,9.0和17.3 cM,该抗病基因可能来源于母本即野生二粒AS846,此基因不同于已有抗白粉病基因,可能为新基因。
A powdery mildew resistance gene, originating from wild emmer（ Triticum dicoccoides Thell. ）accession AS846, was transferred to a common wheat line N9659. N9659 was highly resistant to the prevailing Erysiphegraminisf sp tritici Race in Shaanxi Province. The genetic analysis of N9659 was done in order to determine the inheritance of powdery mildew resistance in N9659. The reciprocal crosses FI between N9659 and the susceptible cuhivar Shaanl60 was highly resistant to the powdery mildew, the ratio of resistant plants and susceptible plants in F2 progeny fitted the expected 3 to 1. 127 F2 plants of the crosses Shaan 160 × N9659 were used for SSR analysis, 3 of 208 wheat SSR primer pairs WMS67, WMS408 and WMS604 generated polymorphic DNA fragments between the resistant plants DNA pools and susceptible plants DNA pools. By analyzing the polymorphic markers in these segregating populations, the SSR locus Xgwin67, Xgwm408, and Xgwm604 located on chromosome 5B were found to be linked to Powdery mildew resistance gene with the estimated genetic distance of 6.7,9.0 and 17.3 cM. The resistance gene derived from the mother Aegilops umbelluata AS846, was different from the resistant genes reported now,so it was problely a new gene.
出处
《华北农学报》
CSCD
北大核心
2008年第3期162-165,共4页
Acta Agriculturae Boreali-Sinica
基金
陕西省重大专项(2006KZ05-G3)
关键词
普通小麦
白粉病
抗性基因
染色体定位
SSR标记
Triticum aestivum
Powdery mildew
Resistance gene
Chromosomal location
SSR marker
