摘要
为筛选与抗白粉病基因Pm13紧密连锁的分子标记,以携带Pm13的抗病品系中大01与感病品系金光588为亲本进行杂交,获得F1、F2分离群体和F2:3家系,利用分离群体分组分析法(BSA)进行SSR标记分析。结果表明,中大01携带的Pm13基因位于3BS染色体,5个SSR标记BE398268、wmc674、cfa2226、gwm533.1和BE471274与Pm13连锁,遗传距离分别为0.5、0.8、1.6、13.2、52.1cM,其中紧密连锁的标记BE398268、wmc674和cfa2226可用于该基因的分子标记辅助选择。
To detect molecular markers closely linked to wheat powdery mildew resistance gene Pm13 ,bulked segregant analysis （BSA）was used to identify microsatellite （simple sequence repeats,SSR）markers in the F2 population from the cross Zhongda 01（resistant）＆#215;Jinguang 588 （susceptible）.The results indicated that Pm13 in Zhongda 01 was located in the 3BS region, linked to five SSR markers BE398268,wmc674,cfa2226,gwm533.1 and BE471274 with the genetic distances of 0.5 cM,0.8 cM,1.6 cM,13.2 cM and 52.1 cM respectively.The molecular markers BE398268,wmc674,and cfa2226 closely linked to Pm13 can be used in maker-assisted selection（MAS）in wheat breeding program for powdery mildew resistance.
出处
《河南农业科学》
CSCD
北大核心
2014年第10期62-66,共5页
Journal of Henan Agricultural Sciences
基金
国家科技支撑计划项目(2011BAD07B02)
河南省教育厅科学技术研究重点项目(12B210003)
