摘要
普通小麦种质N9134含有野生二粒小麦AS846的抗白粉病基因,该种质对陕西省关中地区白粉病流行小种关中4号表现高抗。用高感小麦白粉病的普通小麦品种陕160和陕优225与N9134杂交,F1代对白粉病表现高抗,F2代抗病和感病植株的比例符合3∶1,表明N9134苗期白粉病抗性由1对完全显性基因控制,暂定名为PmAS846。采用66个小麦SSR引物对2个抗感分离群体共176个单株进行分析,发现引物WMS37、WMS67和WMS213的扩增产物在抗感植株DNA池间存在多态性。经分离群体验证,抗病基因PmAS846与小麦染色体5BL上的微卫星位点Xgwm67连锁,遗传距离为20.6 cM,表明PmAS846可能位于5BL上。
A powdery mildew resistance gent, originating from wild cromer （Triticum dicoccoides Thell. ） accession ‘AS846＇, was transferred to a common wheat line N9134. N9134 was highly resistant to the prevailing Erysiphe graminis f. sp. tritici Race Guanzhong 4 in Shaanxi Province. F1 plants of the crosses susceptible cultivars （Shaan 160 and Shaanyou 225） × N9134 were highly resistant to the powdery mildew race and the ratio of resistant and susceptible plants in F2 progeny fitted the expected 3 to 1. The results indicated that powdery mildew resistance of N9134 at seedling stage was controlled by a single dominant gene. The gene is temporarily designated PmAS846. 92 and 84 F2 plants of the crosses Shaan 160× N9134 and Shaanyou 225 × N9134 were used for SSR analysis, respectively. 3 of 66 wheat microsatellite primer pairs WMS67, WMS213 and WMS37 generated polymorphic DNA fragments between the resistant and susceptible plants DNA pods. By analyzing the polymorphic markers in these segregating populations, the microsatellite locus Xgwm67 located on chromosome 5BL was found to be linked to the resistance gene PmAS846 with the estimated genetic distance of 20.6 cM, suggesting this gene might be located on the long arm of chromosome 5B.
出处
《作物学报》
CAS
CSCD
北大核心
2007年第1期163-166,共4页
Acta Agronomica Sinica
基金
the National High Technology Research and Development Program of China (863Program)(2003AA207100) and the Key Technologies R&D Program of Shaanxi Province(2003K03-G1-02)
